江苏大学学报（医学版）Issue(3)：190-194,198,6.DOI:10.13312/j.issn.1671-7783.y140275
TGF-β1介导 NF-κB 信号通路抑制横纹肌肉瘤的肌分化
Mechanism of TGF-β1 mediated NF-κB signal pathway inhibiting myogenic differentiation of rhabdomyosarcoma
摘要
Abstract
Objective:To investigate the mechanism of TGF-β1 mediated NF-κB signal pathway inhibi-ting myogenic differentiation of rhabdomyosarcoma(RMS).Methods:After transfected with TGF-β1 siR-NA-expressing plasmid system,the expression level of Caspase-3 were detected by immunohistochemical staining.The apoptosis of RD cells was assessed by TUNEL assay,and myofilament were explored by trans-mission electron microscope (TEM).After co-culturing with exogenous TGF-β1 ,the expression level of nu-clear factor-kappa B(NF-κB)family members such as p65,p50,p52 and RelB in RD cells were tested by immunofluorescence.The expression level of NF-κB family members in rhabdomyosarcoma paraffin tissues were determined by Immunohistochemistry.Results:Compared with the control group,the expression of Caspase-3 and the positive cells detected by TUNEL assay were increased significantly (P <0.05);TEM showed that structure of myofilament significantly increased in RD cells treated with TGF-β1 siRNA-express-ing plasmid system.After 6 hours for co-culturing with exogenous TGF-β1 (2 ng/mL),RD cells showed sig-nificantly higher positive rate and staining intensity of p65 and p50 than those in control group(P <0.05). The high expression rate of p65 and p50 was found in TGF-β1 -high RMS than that in TGF-β1 -low group (P <0.05).Conclusion:Differentiation inhibition of rhabdomyosarcoma may be related with the TGF-β1 signal transduction mediated by p65 and p50,which is belong to the members of NF-κB family.关键词
横纹肌肉瘤/TGF-β1/NF-κB/肌分化Key words
rhabdomyosarcoma/TGF-β1/nuclear factor-κB/myogenic differentiation分类
医药卫生引用本文复制引用
姜珍,陈枉枉,曹丹,黄封博,房建凯,孙莉,孙茂民,王守立..TGF-β1介导 NF-κB 信号通路抑制横纹肌肉瘤的肌分化[J].江苏大学学报（医学版）,2015,(3):190-194,198,6.基金项目
国家自然科学基金资助项目（81272738，81372867）；江苏省自然科学基金资助项目（BK2011292）；苏州市应用基础研究资助项目 （）
