实用临床医药杂志Issue(7)：7-10,4.DOI:10.7619/jcmp.201407002
茶多酚对人结肠癌细胞株 Caco-2凋亡及RhoA 蛋白活性的影响
Influence of tea polyphenols on the apoptosis of human colon cancer cell line Caco-2 and RhoA protein activity
摘要
Abstract
Objective To explore the influence of tea polyphenols (TP)on the apoptosis of human colon cancer cell line Caco-2 and RhoA protein activity.Methods The passage human colon cancer cell line Caco-2 cells were divided into experimental group and control group.The experimental was added with different concentrations of TP (25,50,100 and 200 μmol /L),while the control group was added with equal amount of culture fluid RPMI-1640.Inhibition of Caco-2 proliferation,ap-optosis indexes and RhoA protein activity within human colon cancer cell line Caco-2 cells were re-spectively detected by methyl thiazolyl tetrazolium (MTT),flow cytometry (FCM)and Pull-down method.Results Different concentrations of TP showed significant inhibitory effects on human colon cancer cell line Caco-2.Pull-down test results showed that TP could reduce the activity of RhoA pro-tein in human colon cancer cell line Caco-2 cells,and with the increasing of concentration,its protein activity decreased.Conclusion TP can promote the apoptosis of human colon cancer cell line Caco-2, which may be achieved by reducing the activity of RhoA protein.关键词
茶多酚/结肠癌/Caco-2/RhoA蛋白Key words
tea polyphenols/colon cancer/Caco-2/RhoA protein分类
医药卫生引用本文复制引用
董丽华,朱旭,姜仕柱,李琪毅..茶多酚对人结肠癌细胞株 Caco-2凋亡及RhoA 蛋白活性的影响[J].实用临床医药杂志,2014,(7):7-10,4.基金项目
三峡大学青年科学基金项目（KJ2011A021）；中国高校医学期刊临床专项资金 （）
