军事医学Issue(5)：388-391,4.DOI:10.7644/j.issn.1674-9960.2014.05.017
利用2，3，5-三苯基氯化四氮唑（TTC）测定细胞活力的方法与应用
Development and application of triphenyltetrazolium chloride (TTC) method for determining the survival of cultured cells
摘要
Abstract
Objective To develop a precise and reliable method to determine mammalian cell survival using the triphe-nyltetrazolium chloride (TTC) solution.Methods Different amounts of TTC solution were added to the cultured cells in order to find the optimal concentration .The correlation between the cell number and the measured optic density value was analyzed.In addition, the TTC method was compared with traditional MTT assay .Results The TTC assay could precisely reveal the viability of cultured cells as well as the MTT method , but the non-specific reaction of the MTT reagent by other antioxidants could result in unreliable overestimation of the cell number .Conclusion The TTC assay can be used to deter-mine the survival of cultured cells more precisely .关键词
TTC/MTT/细胞活力Key words
TTC/MTT/cell survival分类
生物科学引用本文复制引用
何国维,李伟光,王瑞雪,张成岗..利用2，3，5-三苯基氯化四氮唑（TTC）测定细胞活力的方法与应用[J].军事医学,2014,(5):388-391,4.基金项目
国家重点基础研究发展计划（973计划）资助项目（2012CB518200）；国家自然科学基金资助项目（81271206，81100979，31101049，81070741）；国家科技重大专项“十二五”计划资助项目（2012ZX09102301-016）；蛋白质组学国家重点实验室资助项目 （）
