军事医学Issue(7)：527-531,5.DOI:10.7644/j.issn.1674-9960.2014.07.014
一种高保真单向DNA合成方法初步探索
A high-fidelity DNA unidirection synthesis method:preliminary exploration
摘要
Abstract
Objective To establish a simple but quick method to improve the high fidelity of the synthesis of DNA frag -ments.Methods High fidelity DNA unidirection synthesis method (HFUS) was presented and used that involved Phusion DNA polymerase, BsrDⅠrestriction enzymes and λexonuclease.Using the same system at different temperatures , HFUS method synthesized one positive single-strand DNA and several reverse single -stranded DNA one by one into the target DNA fragment.Results Two random sequences DNA fragments of 340 bp and 450 bp were synthesized using HFUS meth-od.Conclusion This article explores a new method for the synthesis of genes .Through the harvest of 450 bp DNA, HFUS may promise to be a new approach to the synthesis of DNA .关键词
DNA片段合成/单向延伸/高保真DNA单向合成法Key words
DNA fragment synthesis/unidirection stretch/high fidelity DNA unidirection synthesis method分类
生物科学引用本文复制引用
吴逊,陈惠鹏,李玉霞,李北平,李炳娟,白东梅,张昕,凌焱,周围,刘刚..一种高保真单向DNA合成方法初步探索[J].军事医学,2014,(7):527-531,5.基金项目
国家自然科学基金资助项目（81000763）；国家科技重大专项资助项目（2012ZX09301003）；国家973计划资助项目 （）
