军事医学Issue(8)：612-616,5.DOI:10.7644/j.issn.1674-9960.2014.08.011
大肠杆菌基因组水平蛋白质-RNA相互作用初步研究
Protein-RNA interactions in Escherichia coli:a genome-wide study
摘要
Abstract
Objective To conduct a pilot study on genome-wide in vivo protein-RNA interactions in E.coli.Methods Bacterial lysate was treated with RNase before the RNA fragments protected by proteins were extracted from treated lysate and used to construct cDNA library that was applied to high-throughput sequencing .Finally, the transcripts bound by proteins were obtained by bioinformatics analysis .Results A total of 3193 transcripts were obtained , including 2234 mRNAs, 47 sRNAs, 39 tRNAs, 11 rRNAs, and 862 intergenic regions .Conclusion Some information of transcripts interacting with proteins in E.coli is acquired , which will facilitate further studies of protein-RNA interactions .关键词
蛋白质-RNA相互作用/sRNA/大肠杆菌/MCAKey words
protein-RNA interactions/sRNA/E.coli/MCA分类
生物科学引用本文复制引用
徐淞,陈垚文,应晓敏,付汉江,田宝磊,宋宜,郑晓飞,李伍举..大肠杆菌基因组水平蛋白质-RNA相互作用初步研究[J].军事医学,2014,(8):612-616,5.基金项目
国家973计划资助项目（2010CB912801）；国家自然科学基金资助项目 （）
