军事医学Issue(8)：617-620,625,5.DOI:10.7644/j.issn.1674-9960.2014.08.012
Tim-3抗体的生物学活性及应用研究
Biological activity and application study of a monoclonal antibody against human Tim-3
摘要
Abstract
Objective To develop a human Tim-3 specific monoclonal antibody and evaluate its biological activity and possible use in clinical diseases associated with dysregulated Tim-3 expression .Methods The BALB/c mice were immu-nized by conventional method, and positive clones were used to develop anti-human Tim-3 antibody, the binding and neutralization activities of which in vitro and in vivo were investigated.Results ①A monoclonal antibody (clone L3D) which could specifically bind to human Tim-3 protein in ELISA assay was obtained and the subtype of the monoclonal antibody was IgG2a .②Flow cytometry indicated that the monoclonal antibody could bind to Tim-3 expressed in human U937 cells.This antibody also showed a cross activity to mice′Tim-3.③The monoclonal antibody inhibited the apoptosis of THP1 cells induced by Gal-9, the ligand of Tim-3.④Injection of Tim-3 antibody exacerbated sepsis in mice as marked by the decreased survival rate and increased expression of pro-inflammatory cytokines .Conclusion An anti-human Tim-3 monoclonal antibody is successfully obtained.The excellent binding and neutralization activities of this antibody enable it to be widely used in clinical diseases associated with deregulated Tim-3 expression .关键词
Tim-3/抗体,单克隆/L3D/U937细胞/炎性因子/脓毒症Key words
Tim-3/antibodies,monoclonal/L3D/U937 cells/inflammatory factors/sepsis分类
生物科学引用本文复制引用
侯春梅,韩根成,沈倍奋,黎燕,郎小玲,肖燕,郑明克,蒋兴伟,肖鹤,陈国江,王仁喜,冯健男..Tim-3抗体的生物学活性及应用研究[J].军事医学,2014,(8):617-620,625,5.基金项目
国家973计划资助项目（2013CB530506）；国家自然科学基金资助项目 （）
