军事医学Issue(11)：860-862,3.DOI:10.7644/j.issn.1674-9960.2014.11.005
E-钙黏蛋白和N-钙黏蛋白启动子的构建及活性测定
Construction and activity characterization of E-cadherin and N-cadherin promoters
摘要
Abstract
Objective To clone E-cadherin and N-cadherin promoters and insert them into a luciferase reporter gene vector, and to characterize the promoter activity of E-cadherin and N-cadherin.Methods E-cadherin and N-cadherin pro-moter were cloned into pGL 4-basic.The resulting plasmids were determined by DNA sequencing .The promoter activity was analyzed in breast cancer cell line ZR 75-1 and hepatocarcinoma cell line HepG 2.Results DNA sequencing showed that the sequences of the cloned promoter regions were correct .Analysis of the reporter gene activity indicated that the E-cad-herin and N-cadherin promoters had the highest transcriptional activity in ZR 75-1 and HepG2 cells.Conclusion The E-cadherin and N-cadherin promoter genes are cloned successfully , contributing much to screening transcription factors that regulate E-cadherin and N-cadherin expression .关键词
E-钙黏蛋白/N-钙黏蛋白/启动子/荧光素酶报告基因Key words
E-cadherin/N-cadherin/promoter/luciferase reporter gene分类
生物科学引用本文复制引用
周蕾,张亚楠,刘婕,丁丽华,叶棋浓..E-钙黏蛋白和N-钙黏蛋白启动子的构建及活性测定[J].军事医学,2014,(11):860-862,3.基金项目
国家自然科学基金资助项目（31071174，81272231）；北京科技新星资助项目 （）
