军事医学Issue(12)：948-951,4.DOI:10.7644/j.issn.1674-9960.2014.12.007
重组尿激酶原治疗对大鼠肺血栓栓塞症后血浆纤溶因子的影响
Changes of plasma fibrinolytic factors in pulmonary thromboembolism rats after rce-combinant prourokinase treatment
毛文苹 1王辰 2杨媛华 1庞宝森 1欧阳松云3
作者信息
- 1. 首都医科大学附属北京朝阳医院，呼吸与危重症医学科，北京 100043
- 2. 北京呼吸疾病研究所，国家卫生和计划生育委员会 原卫生部 中日友好医院，北京 100029
- 3. 郑州大学第一附属医院，郑州 450052
- 折叠
摘要
Abstract
Objective To observe the changes in fibrinolytic factors in rats with pulmonary thromboembolism (PTE) after recombinant prourokinase ( rPro-UK) treatment and its significance .Methods PTE was induced in male Sprague-Dawley (SD) rats by injecting heated 125iodine-labeled fibrinogen(Fib) autologous thromboemboli into external jugular veins.Twenty-eight rats were randomly assigned into following groups (7 rats each):①healthy control group;②PTE 5 d group,the rats in which were sacrificed at 5 d after the PTE model was made; ③ PTE3d receiving rPro-UK thrombolytic treament groups including multibolus treatment sub group ( rPro-UK was given in 1 mg/kg on the post-PTE third day followed by 2 consecutive days of a lower dose 0.25 mg/kg and rats were sacrificed 2 h after the last injection at the same time as PTE5d group) and single bolus treatment sub group ( rPro-UK was given in 1 mg/kg on the post-PTE third day followed by 2 consecutive days of 0.5 ml saline and rats were sacrificad at the same time as the former group ).The rats were quickly sacrificad at the fixed time through carotid bleeding and plasma samples were reserved for analysis of uroki -nase-type plasminogen activator (u-PA), urokinase-type plasminogen activator receptor (u-PAR), fibrinogen (Fib) andα2-antiplasmin (α2-AP) .Results ①Plasma concentrations of u-PA and u-PAR were increased were significantly in rPro-UK multibolus treatment sub group than in PTE 5 d group(Pu-PA <0.05,Pu-PAR <0.01)and rPro-UK single bolus treatment sub group(Pu-PA <0.01,Pu-PAR <0.05),correlated with the thrombolysis rate in rPro-UK multibolus treatment sub group (ru-PA =0.766,P<0.05;ru-PAR=0.785,P<0.05).② No difference of plasma Fib and α2-AP was seen between Pro-UK treatment groups and PTE 5 d group(P >0.05).Conclusion ① Plasma levels of endogenous u-PA and u-PAR are increased at different time points after PTE and are further enhanced after Pro-UK treatment, which promotes endogenous fibrinolysis and thrombus lysis .This is probably related to increased synthesis and secretion of endothelial cells which may be a key thrombolytic mechanism of Pro-UK.②Absence of systemic activation of the fibrinolytic system in Pro-UK multibo-lus treatment sub group means that the regimen is feasible and Pro-UK is fibrin specific .关键词
肺血栓栓塞症/重组尿激酶原/尿激酶型纤溶酶原激活物/尿激酶型纤溶酶原激活物受体/纤溶Key words
pulmonary thromboembolism/prourokinase ,recombinant/urokinase type plasminogen activator/urokinase plasminogen activator receptor/fibrinolysis分类
医药卫生引用本文复制引用
毛文苹,王辰,杨媛华,庞宝森,欧阳松云..重组尿激酶原治疗对大鼠肺血栓栓塞症后血浆纤溶因子的影响[J].军事医学,2014,(12):948-951,4.
