军事医学Issue(12)：957-961,5.DOI:10.7644/j.issn.1674-9960.2014.12.009
温肾生精饮对环磷酰胺致小鼠睾丸氧化损伤的保护作用
Protective effect of Wenshen Shengjing Decoction on cyclophosphamide induced testicular oxidative damage in mice
摘要
Abstract
Objective To investigate the protective effect and the possible mechanism of Wenshen Shengjing Decoction (WSSJD)(including Cornu Cervi Nippon Parvum , Panax ginseng, Cynomorium songaricum, Cistanche deserticola,Radix Astragali, Epimedium brevicornum, Angelica sinensis.) on cyclophosphamide induced testicular oxidative damage in mice . Methods Eight-week-old male Kunming mice were assigned .Mice in normal control group received intraperitoneally nor-mal saline, and mice in Western medicine group ,WSSJD group and model group were injected intraperitoneally with cyclo-phosphamide [80 mg/(kg・ d) ]for five days.Then mice in Western medicine group and WSSJD group were gavaged with clomifene citrate or WSSJD for 30 days consecutively .The epididymal sperm count and sperm viability were recorded , and the structure of seminiferous epithelium was observed .The content of malondialdehyde (MDA) and glutathione (GSH), and activities of glutathione peroxidase ( GSH-Px) and catalase ( CAT) in testicular tissue were detected .Immunohisto-chemical technique was used to detect the expression of survivin in spermatogenic cells .The apoptosis of spermatogenic cells in the testes was detected by TUNEL assay .Results Compared with model and Western medicine groups ,the epidid-ymal sperm count and sperm viability rate , the development of seminiferous epithelium , and the activities of GSH-Px and CAT significantly increased , while the MDA content significantly decreased in WSSJD group .The expression of survivin in spermatogenic cells was significantly improved .The apoptotic indexes of seminiferous tubules and spermatogenic cells significantly decreased in WSSJD group .Conclusion These results suggest that WSSJD can significantly improve the impaired reproductive function induced by cyclophospamide in mice by enhancing the antioxidative capability in testes and reducing the apoptosis of spermatogenic cells .关键词
温肾生精饮/环磷酰胺/睾丸/氧化损伤/生存素/凋亡Key words
Wenshen Shengjing Decoction/cyclophosphamide/testis/oxidative damage/survivin/apoptosis分类
医药卫生引用本文复制引用
潘晓燕,李康,王喜艳,王雪楠,孙占轩,严之贞,林寿想,范佳佳,李质馨..温肾生精饮对环磷酰胺致小鼠睾丸氧化损伤的保护作用[J].军事医学,2014,(12):957-961,5.基金项目
吉林省科技发展计划重点攻关项目（20140204033YY）；吉林省中医药管理局中医药科技项目（2012－165）；吉林市科技发展计划资助项目（2013625023）；山东省高等学校科技计划项目（J13LL04）；吉林医药学院大学生科研基金［吉医学科字（2012）第03号］；2014年吉林省大学生创新创业训练计划项目 （）
