军事医学Issue(2)：136-139,4.DOI:10.7644/j.issn.1674-9960.2015.02.014
2单位冰冻红细胞自动化洗涤程序研究
Protocol of automatically processing 2 U frozen red blood cells
摘要
Abstract
Objective To improve the processing protocol of the frozen erythrocyte ( RBC) washing machine of the dialysis type to enhance its efficiency of treating the 2 U frozen RBCs .Methods The frozen RBC washing machine for dialysis was used to deglycerolize the 2 U frozen RBCs after thawing .Measures including adjustment of the Pump speed , washing buffer adding and solution osmolarity were taken to decrease the processing time .Hemoglobin(Hb) content, Hb recovery, residual glycerol, free Hb content, deforming ability, and apoptosis of the deglycerolized RBCs were assessed to evaluate the efficiency of the improved protocol .Results As for the cells processed respectively by the previous and improved protocols, contents of Hb were (35.03 ±4.20) g and (53.82 ±2.08) g, respectively; Hb recoveries were (61.10 ±8.46)% and (80.22 ±3.73)%,respectively.Osmolarities representing residual glycerol were (307.00 ± 14.50)mOsm and (322.00 ±29.00)mOsm.Free Hb values were (0.74 ±0.04) and (0.80 ±0.08)g/L.The processing time was (1.64 ±0.31) and (1.04 ±0.16) h,respectively.There were significant differences in Hb content , Hb recovery, and deforming ability between the cells processed by the two protocols respectively .However , there were no significant changes in glycerol contents , free Hb, and apoptosis extent between the cells processed by the two protocols .Conclusion The innovated protocol can improve the quality of washed frozen RBCs , decrease the processing time and enhance the efficiency of the machine.关键词
透析式冰冻红细胞洗涤机/2单位冰冻红细胞/洗涤程序/低温保存Key words
frozen RBC washing machine ,dialysis type/2 U of frozen red blood cells/washing protocol/cryopreserva-tion分类
医药卫生引用本文复制引用
王艳,刘敏霞,成海,李文静,王捷熙,杜为,韩颖,王东根,蒋学兵..2单位冰冻红细胞自动化洗涤程序研究[J].军事医学,2015,(2):136-139,4.基金项目
全军医学科研“十二五”重大项目（AWS135004）；海军“十二五”重点课题资助项目 （）
