军事医学Issue(3)：165-168,4.DOI:10.7644/j.issn.1674-9960.2015.03.002
血液制品病毒灭活／去除技术平台的建立与应用
Establishment and application of viral inactivation/removal technology for blood products
摘要
Abstract
Objective To establish viral inactivation/removal techniques for blood products , and apply them to inacti-vation/removal process validation of blood products .Methods Enveloped and non-enveloped model viruses were propaga-ted.Viral inactivation/removal techniques for blood products ,including solvent/detergent (S/D) treatment, low pH incuba-tion, dry heat method, pasteurization,and nanofiltration, were established.The virus titer was evaluated using cytopathic effects ( CPE) and Spearman and Karber method .The viral inactivation/removal techniques were believed to be effective when LRV≥4.These techniques were used in viral inactivation /removal validation of blood products .Results Enveloped model viruses were inactivated through S/D treatment and the low pH incubation method .Enveloped and non-enveloped model viruses were inactivated through dry heat and pasteurization .Within a certain range of filtration capacity , PPV was removed through nanofiltration .Conclusion The established viral inactivation/removal techniques can be used in viral inactivation/removal process validation of blood products , which can improve viral safety of blood products .关键词
血液制品/病毒灭活/消毒Key words
blood products/viral inactivation/disinfection分类
医药卫生引用本文复制引用
尹惠琼,王蕊,朱凤宣,王建国,章金刚..血液制品病毒灭活／去除技术平台的建立与应用[J].军事医学,2015,(3):165-168,4.基金项目
国家863计划资助项目 （）
