军事医学Issue(3)：179-183,5.DOI:10.7644/j.issn.1674-9960.2015.03.005
水痘－带状疱疹病毒快速微量中和试验的建立与初步应用
Establishment and preliminary application of a rapid microneutralization test for screening varicella-zoster virus special hyperimmune globulin in plasma donations
摘要
Abstract
Objective To promote the progress in varicella-zoster virus (VZV) immunoglobulin preparation,a rapid microneutralization test ( RMNt) was set up for screening plasmapheresis donations with high titers of special neutralizing antibodies to VZV.Methods With reference to the VZV immunoglobulin (VZIG) preparation standard of FDA and VZIG international unit ( IU) , a screening standard was formulated; the amount of virus was analyzed to determine the optimal conditions for RMNt;screening technology was established and the IU was introduced as quality control ;twenty samples of apheresis plasma and fifteen samples of pooled plasma were diluted at 1∶2 to 1∶256 and tested by RMNt respectively;and the sensitivity of RMNt was also analyzed by the commercial ELISA kit .Results Plasma samples that were diluted at 1∶16 and had a titer more than 0.4 U/ml could be used in the production of VZIG .1500 PFU/ml titers of virus in RMNt pro-duced readable results in plasma screening .Eight apheresis plasma samples tested met the screening standard , but none of the pooled samples tested positive .RMNt had a good linear relationship with ELISA (r=0.895 24,P<0.0001).Conclu-sion The sensitivity, throughput and operability of the established RMNt can be used in the screening of plasma donations as key techniques for the production of VZIG .关键词
水痘-带状疱疹病毒/免疫球蛋白类/快速微量中和试验/血浆Key words
varicella-zoster virus/immunoglobulins/rapid microneutralization test/plasma分类
医药卫生引用本文复制引用
王卓,吕茂民,赵雄,马玉媛,赵媛媛,章金刚..水痘－带状疱疹病毒快速微量中和试验的建立与初步应用[J].军事医学,2015,(3):179-183,5.基金项目
国家863计划资助项目 （）
