军事医学Issue(4)：266-270,301,6.DOI:10.7644/j.issn.1674-9960.2015.04.008
多氯联苯与高脂联合暴露SD大鼠的代谢组学研究
Metabonomics analysis of effects induced by PCBs and high fat diet combined expo-sure in SD rats
摘要
Abstract
Objective To explore the effect on metabolism of glucose and lipids, potential toxicity mechanism and possible biomarker candidates by analyzing urine metabonome changes of rats after oral administration of polychlorinated biphenyl congeners( PCBs) and high fat diet alone or in combination.Methods Male SD rats were divided randomly into control group,high fat diet group, PCBs group and combination group of PCBs and high fat diet.Urine samples were collected after 6-week treatment, 1 H NMR spectra were performed and analyzed by principal component analysis ( PCA) . Results The PCA scores plot of urine 1 H NMR data showed that the combined group could be easily distinguished from the other three groups, suggesting great difference in metabolism.The loading plot of the PCA revealed significant increase in the levels of lactate, glucose, creatine, 2-hydroxy-isovaleric acid and reduction in the levels of citrate, succinate, taurine, hippurate and trimethylamine oxide ( TMAO) in the combined exposure group after six-week exposure.Conclusion The altered levels of metabolites induced by combined exposure of PCBs and high fat diet may be related to the injury to mitochondrial function, reduction of energy metabolism in tricarboxylic acid cycle (TAC).These effects possibly lead to perturbations in the metabolism of glucose, lipid and amino acids.The altered metabolites may be considered biomarker candidates of toxicity induced by exposure to PCBs and high fat diet.关键词
多氯联苯/高脂饮食/2型糖尿病/代谢组学/主成分分析Key words
polychlorinated biphenyl congeners/high fat diet/type 2 diabetes/metabonomics/principal component analysis分类
医药卫生引用本文复制引用
李致远,彭双清,王以美,薛耀明,方毅,颜贤忠,贾栗,张廷芬,华辰凤,张琪..多氯联苯与高脂联合暴露SD大鼠的代谢组学研究[J].军事医学,2015,(4):266-270,301,6.基金项目
国家重点基础研究发展计划资助项目 （）
