军事医学Issue(5)：334-338,363,6.DOI:10.7644/j.issn.1674-9960.2015.05.004
xCT调节乳腺癌细胞转移的作用机制研究
Molecular mechanism of metastasis in breast adenocarcinoma regulated by xCT
摘要
Abstract
Objective To investigate the mechanism of xCT on tumor metastasis in breast cancer cell MDA-MB-231. Methods Wound scratch assay and Transwell assay were performed to evaluate the effect of disruption and knockdown of xCT on cell migration and cell invasion in breast cancer cell MDA-MB-231 .Western Blot and RT-PCR were used to detect the expression levels of autophagy and EMT related markers in breast cancer cell MDA-MB-231 after treatment with sulfasalazine (SASP), an inhibitor of xCT activity and SLC7A11-RNAi.Results Both the scratch assay and the transwell migration assay showed that inhibition of xCT reduced the motility of MDA-MB-231 .The expression level of autophagy related protein LC3-Ⅱ/LC3-Ⅰwas elevated, the protein level of transcription factor Snail was down-regulated, while the mRNA level of Snail did not change in xCT inhibited MDA-MB-231 cells compared with MDA-MB-231 cells.Epithelial marker E-cadherin was up-regulated but mesenchymal marker Vimentin was down-regulated when xCT was deficient.Con-clusion Our current studies show that xCT is an endogenous regulator of tumor growth and metastasis in MDA -MB-231 and the expression level of xCT determines the phenotypes of MDA-MB-231 cells in invasion and migration in vitro.Inhibition of xCT can activate autophagy , induce the degradation of Snail ,and attenuate the EMT process in highly metastatic MDA-MB-231 cells.关键词
乳腺癌细胞株MDA-MB-231/自体吞噬/EMT/SASP/RNA干扰Key words
breast cancer cell MDA-MB-231/autophagy/EMT/SASP/RNAi分类
生物科学引用本文复制引用
郑雪婷,赵飞,赵瑞,赵立平,乔海晅..xCT调节乳腺癌细胞转移的作用机制研究[J].军事医学,2015,(5):334-338,363,6.基金项目
天津市自然科学基金资助项目 （）
