军事医学Issue(6)：443-447,452,6.DOI:10.7644/j.issn.1674-9960.2015.06.008
长链非编码RNA HOTTIP对宫颈癌细胞增殖、迁移及侵袭的影响
Effect of lncRNA HOTTIP on proliferation, migration and invasion of cervical cancer cells
刘芳 1李立安 2张欢 3张唯一 4吴晓洁 3郗永义 4周艳荣 4陈红星 4林艳丽4
作者信息
- 1. 安徽大学生命科学学院，合肥 230601
- 2. 军事医学科学院生物工程研究所，北京 100071
- 3. 解放军总医院妇产科，北京 100853
- 4. 军事医学科学院生物工程研究所，北京 100071
- 折叠
摘要
Abstract
Objective To investigate the potential biological effect of long non-coding RNA( lncRNA) HOXA transcript at the distal tip( HOTTIP) on proliferation, migration and invasion of cervical cancer cells.Methods HOTTIP small interference RNA(siRNA) was transfected into HeLa and C-33A cervical cancer cell lines, with negative siRNA as a control.qPCR assay was performed to confirm the knock-down of the level of HOTTIP.CCK8 assay and colony-forming unit (CFU) assay were performed to evaluate the effect of HOTTIP knock-down on HeLa and C-33A cell proliferation.Wound healing assay was performed to evaluate the effect of HOTTIP knock-down on HeLa and C-33A cell proliferation and migration.Tumor invasion assay was used to evaluate the effect of HOTTIP knock-down on HeLa and C-33A cell invasion. Results The expression level of HOTTIP was efficiently knocked down by siRNA 48 h post transfection.The results of CCK8 assay and CFU assay showed that HOTTIP knock-down significantly decrease of cervical cancer cell proliferation. Wound healing assay result indicated that HOTTIP knock-down obviously suppressed cervical cancer cell proliferation and migration.Tumor invasion assay results demonstrated that HOTTIP knock-down significantly suppressed cervical cancer cell invasion.Conclusion HOTTIP levels in HeLa and C-33A cervical cancer cell lines can be efficiently knocked down with the siRNA strategy, and the HOTTIP knock-down can significantly suppress the tumor characteristics of cervical cancer cells, including the ability of proliferation, migration and invasion.关键词
lncRNA/HOTTIP/宫颈肿瘤/细胞增殖/细胞运动/肿瘤浸润Key words
lncRNA/HOTTIP/uterine cervical neoplasms/cell proliferation/cell movement/neoplasm invasiveness分类
医药卫生引用本文复制引用
刘芳,李立安,张欢,张唯一,吴晓洁,郗永义,周艳荣,陈红星,林艳丽..长链非编码RNA HOTTIP对宫颈癌细胞增殖、迁移及侵袭的影响[J].军事医学,2015,(6):443-447,452,6.
