军事医学Issue(6)：453-455,479,4.DOI:10.7644/j.issn.1674-9960.2015.06.010
PPARβ激动剂对肥大心肌细胞蛋白合成及IL-1β表达的影响
Regulation of PPARβactivation on protein synthesis and IL-1βexpression in hypertrophic cardiomyocytes
摘要
Abstract
Objective To investigate the effect of activation of peroxisome proliferator-activated receptor β/δ ( PPARβ/δ)on protein synthesis and expression of angiotensin (Ang)Ⅱ-induced hypertrophic myocytes(MC) in vitro.Methods Hypertrophy in neonatal rat cardiac MC culture was established with AngⅡ, then the effect of GW0742 on hypertrophy was detected.The synthetic rate of protein in MC was detected by 3 H-leucine incorporation.mRNA and protein expression of atrial natriuretic IL-1βwas measured by reverse transcription-polymerase chain reaction ( RT-PCR) and Western-blotting. Results and Conclusion GW0742 could reduce the synthetic rate of protein in hypertrophic MC while down-regulating the mRNA and protein expression of IL-1β, but no changes were observed after treatment with DMSO.The result demonstrated that activation of PPAR beta/delta inhibited cardiac hypertrophy in vitro and this effect might be related to inflammatory factors.关键词
过氧化物酶体增殖物活化型受体/心肌细胞肥大/白细胞介素-1β/血管紧张素ⅡKey words
peroxisome proliferator-activated receptor/cardiac hypertrophy/interleukin-1β/angiotensinⅡ分类
医药卫生引用本文复制引用
盛莉,叶平,韩春光,刘永学..PPARβ激动剂对肥大心肌细胞蛋白合成及IL-1β表达的影响[J].军事医学,2015,(6):453-455,479,4.基金项目
国家重点基础研究发展计划资助项目 （）
