江西农业大学学报Issue(5)：958-964,7.
AgNO3对银杏胚愈伤组织诱导及分化的影响
Influence of AgNO3 on Induction and Differentiation of Ginkgo biloba Embryo Callus
摘要
Abstract
To study the effect of AgNO3 on Ginkgo zygotic embryos at different developing stages,embryos at three developing stages were taken as explants with different concentration (0-5 mg/L) of AgNO3 .The re-sult showed that:AgNO3 affected the rate of callus significantly:the maximum rate of early heart-shape or tor-pedo-type embryos was 87%at 1 mg/L,that of the early cotyledon embryos was 77%at 0.5 mg/L,that of the mature cotyledons was 98%at 0.1mg/L.Inter-generational treatment of AgNO3 showed a significant impact on differentiation of the early cotyledon embryos.After 90 days,the tissue differentiated leaves and embryo ana-logues at 1.0 mg/L,short dry leaves at 3.0mg/L.while with long-term treatment of AgNO3 ,the tissue differen-tiated bud at 0.1 mg/L after 50 days.Heart-shape embryos were cultured in a dark place,Ag+couldn’ t affect the rate of callus,and treated with activated carbon the cotyledon embryos,couldn’ t produce callus,the hypo-cotyl induction rate was 71%at 0.1mg/L,the callus were white and lucid.Comprehensive analysis shows that AgNO3 can improve the rate of callus and affect the differentiation of Ginkgo embryos significantly.关键词
AgNO3/银杏胚/愈伤组织/分化Key words
AgNO3/Ginkgo embryos/callus/differentiation分类
生物科学引用本文复制引用
秦小舒,曹福亮,逯岩,郁万文,林芳芝..AgNO3对银杏胚愈伤组织诱导及分化的影响[J].江西农业大学学报,2014,(5):958-964,7.基金项目
江苏省自然科学基金（BK2010019）和国家自然科学基金 （）
