江西农业大学学报Issue(5)：965-970,6.
木奈叶片乙醇酸氧化酶基因全长 cDNA的分离与表达研究
Isolation of Full-length cDNA of GLO from Pranus salicina Leaf and its Expression
摘要
Abstract
Glycolate oxidase ( GLO) is a key enzyme in photorespiration and catalyzes the oxidation of gly-colate into glyoxylate with an equimolar amount of H2 O2 ,which is related with induction of disease resistance in plant.A clone,named PsGLO,was separated from cDNA library prepared from five different growth and de-velopment periods of leaf of Prunus salicina.Sequence analysis showed that PsGLO was 1 531 bp in length with a 1 122 nucleotides ORF that putatively encoded a protein with 374 amino acids.Multiple sequence alignments and phylogenetic tress analyses showde that GLO was clustered with Gossypium hirsutum GLO and showed high similarity (90%) in amino acid sequences.Quantitative real-time PCR analysis showed that the expression of PsGLO was observed in all different stages tested here including leafbud,unfoldedleaf,spire,climax leaf,old leaf and the highest expression level was found in old leaves,but the lowest in leafbud.关键词
木奈(Prunus salicina Lindl.var.cordata J.Y.Zhang et al.)/乙醇酸氧化酶/全长cDNA/表达分析Key words
Prunus salicina/glycolate oxidase/full-length cDNA/expression analysis分类
农业科技引用本文复制引用
赵利,陈桂信,潘东明,王玉珍,吕恃衡,姜翠翠..木奈叶片乙醇酸氧化酶基因全长 cDNA的分离与表达研究[J].江西农业大学学报,2014,(5):965-970,6.基金项目
科技部国家科技支撑计划项目（2007BAD07B01）、福建省种业创新与产业化工程项目和福建农林大学创新团队项目 （）
