江西农业大学学报Issue(5)：1127-1131,5.
太子参根际尖孢镰刀菌绝对荧光定量检测方法的建立及其应用
Establishment and Application of qRT-PCR Method for Quantification of Fusarium oxysporum f.sp.in Pseudostellaria heterophylla Rhizosphere
摘要
Abstract
The specific primers of Fusarium oxysporum f.sp.were designed and the molecular quantitative detection method was built to be used to quantify and analyze the Fusarium oxysporum f.sp.in rhizosphere soil under monoculturally cropped Pseudostellaria heterophylla for different years.The results showed that ( 1 ) the amplified positive strip was 106 bp;the minimum detectable amount of Fusarium oxysporum f.sp.DNA was 0.000 012 ng/μL with this method and the amplification results of Fusarium graminearum,Fusarium solani,Aspergillus flavus,bacillus amyloliquefaciens were all negative,(2) the quantifying results of Fusarium oxyspo-rum f.sp.with this method suggested that the number of Fusarium oxysporum f.sp.increased with the monocul-ture years.This detecting method is of accuracy,high sensitivity and easy accessibility,which realizs the leap from qualitative to quantitative.It offers the methods and foundation for analysis of this pathogen in the rhizo-sphere micro ecology of Pseudostellaria heterophylla,and illuminates the reason for large-scale occurrence of the disease in successively cropped Pseudostellaria heterophylla.关键词
太子参/连作障碍/根际微生态/尖孢镰刀菌/qRT-PCRKey words
Pseudostellaria heterophylla/successive cropping obstacle/rhizosphere microecology/Fusari-um oxysporum/qRT-PCR分类
农业科技引用本文复制引用
赵永坡,初雷霞,岳开华,李振方,林文雄..太子参根际尖孢镰刀菌绝对荧光定量检测方法的建立及其应用[J].江西农业大学学报,2014,(5):1127-1131,5.基金项目
国家重点基础研究发展计划（973项目2012CB126309）和国家自然科学基金项目 （）
