江西农业大学学报Issue(5)：1132-1138,7.
GM-CSF与生长抑素共表达基因疫苗的构建和鉴定
Construction and Identification of Co-expression Gene Vaccine of GM-CSF and Somatostatin (SS)
摘要
Abstract
With gene vaccine pVGS/2SS-asd for template,target fragments of granulocyte-macrophage colony-stimulating factors ( GM-CSF) and somatostatin ( SS) gene contained the S gene of HBsAg were ob-tained by molecular biology.With co-expression plasmid pIRES for vector, GM-CSF and SS gene fragments were cloned to MCSA( multiple cloning site A) and MCS B ( multiple cloning site B) of pIRES by genetic engi-neering.Co-expression gene vaccine of GM-CSF and SS ( pIRES-GM-CSF/2SS) was constructed.It was de-signed to expression protein of GM-CSF and protein of SS gene simultaneously.pIRES-GM-CSF/2SS was i-dentified with enzyme digestion and sequencing.The results showed that co-expression gene vaccine pIRES-GM-CSF/2SS was successfully constructed,which provides a material foundation for immunomodulation of GM-CSF on DNA vaccine.关键词
粒细胞-巨噬细胞集落刺激因子(GM-CSF)/生长抑素(SS)/共表达基因疫苗Key words
granulocyte-macrophage colony-stimulating factor(GM-CSF)/somatostatin(SS)/co-ex-pression gene vaccine分类
生物科学引用本文复制引用
魏金钢,韩晓萍,刘英俊,兰芳菲,梁爱心,吴红翔,舒邓群..GM-CSF与生长抑素共表达基因疫苗的构建和鉴定[J].江西农业大学学报,2014,(5):1132-1138,7.基金项目
江西省科技厅科技支撑计划（20112BBF60020）和江西农业大学博士启动基金项目 （）
