江西农业大学学报Issue(6)：1325-1331,7.DOI:10.13836/j.jjau.2014211
一株健康仔猪肠道乳酸菌的筛选鉴定与饲喂安全性的研究
Characterization of An Enterococcus faecium Strain Isolated from Healthy Piglet Intestinal Tract and Its Feeding Safety as Potential Probiotic
摘要
Abstract
A strain of lactic acid bacterium was screened from the intestinal tract of a healthy piglet.It’s i⁃dentification was based on bacterial culture,physiological and biochemical tests and 16S rDNA sequence analy⁃sis.Its potential probiotic feature was evaluated by its growth curve in MRS and its functional properties,inclu⁃ding antagonistic activity against pathogenic bacteria,survival form gastrointestinal tract conditions ( acidity and bile),antibiotic susceptibility and safety in chicken feeding.A strain WLEF-01 was identified as Enterococcus faecium.It exhibited rapid growth in MRS at 37℃ and entered the logarithmic phase after incubation 0.5 h.The generation time of WLEF-01 was 20 min and it reached 1.5×1011cfu/ mL in the stationary phase.The strain se⁃creted acid to the medium making pH 6.8 to 4.5 and showed better resistance to 1% bile salted and lower pH4.WLEF-01 revealed antagonism against pathogenic E.Coli and S.aureus to some degree.Fed to chicken from 0to 12 weeks,WLEF-01 2×107cfu/ g had no bad effects,which suggested that the strain is safe.From the resultsobtained,it can be concluded that WLEF-01 isolated from piglet intestinal tract is a potential probiotic strainfor feeding additive.关键词
屎肠球菌/理化特性/16s rDNA/饲喂安全性/益生菌Key words
Enterococcus faecium/physical and chemical properties/16s rDNA/feeding safety/probiotic分类
生物科学引用本文复制引用
曹珂,林丽萍,陈于陇,卢新民,叶明娥,吴国平..一株健康仔猪肠道乳酸菌的筛选鉴定与饲喂安全性的研究[J].江西农业大学学报,2014,(6):1325-1331,7.基金项目
江西省科技支撑计划和教育厅基金项目（20112BBF60018，GJJ12224）、广东省农业科学院农业部功能食品重点实验室开放基金项目（201351）和江西农业大学大学生创新创业训练计划项目 （）
