江西农业大学学报Issue(2)：269-277,9.DOI:10.13836/j.jjau.2015040
黑暗条件下木薯叶绿体比较蛋白质组学初步研究
A Primary Study of Comparative Proteomics of Chloroplast in Cassava under Dark Condition
摘要
Abstract
The chloroplast of the cassava cultivar ‘South China 8’ ( SC8) was used to extract the chloro⁃plast protein with an improved phenol(BPP) method,and these proteins expressed profiles under dark condi⁃tion were determined with 1⁃DE and 2⁃DE.The obtained typical 2⁃DE gels were analyzed by ImageMaster soft⁃ware to determine the differential expressed protein spots,and some of them were manually excised for protein in⁃gel digestion and subsequently identified by MALDI⁃TOF MS. The main results included:high quality pro⁃teins were obtained from chloroplast in cassava using phenol method;high resolution of 2⁃DE reference maps with good reproducibility were generated for proteomics;the comparison of the 2⁃DE gels for chloroplast of the 1,3,5 day under dark treatment found 186 differential protein spots;twenty⁃eight proteins were finally identi⁃fied by MALDI⁃TOF MS,which were involved in photosynthesis,carbon fixation,energy metabolism,proline metabolism,stress defense,and so on.This study represents a comprehensive dynamic protein profile and light⁃regulated network of SC8 cassava chloroplast under dark treatment.关键词
木薯叶绿体/暗处理/比较蛋白质组学/质谱Key words
cassava chloroplast/dark treatment/comparative proteomics/mass spectrometry分类
农业科技引用本文复制引用
徐兵强,贺庭琪,王力敏,王丹,孙勇,王旭初,郭安平..黑暗条件下木薯叶绿体比较蛋白质组学初步研究[J].江西农业大学学报,2015,(2):269-277,9.基金项目
国家重点基础研究发展计划（973计划）项目（2010CB12660-2，5）和海南省自然科学基金项目 （）
