江西农业大学学报Issue(2)：333-338,6.DOI:10.13836/j.jjau.2015051
山女鳟水霉病病原的分离鉴定及其生物学特性
Identification and Biological Characteristics of a Pathogenic Saprolegnia sp. from Oncorhynchus masou masou
摘要
Abstract
The isolation of the fungal strain from the Oncorhynchus masou masou lesion site through tradi⁃tional methods is named SN, and its biological characteristics. Observation of the strain attached to rapeseed showed that its hyphae was diaphanous tubular,without diaphragm and moderately branched;clavate sporangia produced at the top of the hyphae,mature zoospores were discharged from the sporocyst rapidly.New sporangia generated from the base of old sporangia by the way of internal proliferation or by basipetalous succession.In a suitable growth environment intermediate spherical or irregularly shaped chlamydospores oppeared at the top of the hyphae.Homology comparison of the ITS rDNA sequences of Saprolegnia sp.in GenBank,showed its closest relationship with Saprolegia salmonis ( GenBank accession number:EU551152) Therefore,combined with mor⁃phological characterization with phylogenetic analysis based on ITS rDNA sequence,strain SN was identified as Saprolegnia salmonis.This study investigated the effects of different growth conditions on the biological charac⁃teristics of strains.The strain SN could grow at 10-30 ℃,pH 5-11 and NaCl 0.5%-2%,and its optimum growing temperature,pH and NaCl were 25℃,7,0.5%,respectively.The pathogen of Oncorhynchus masou ma⁃sou saprolegniasis was isolated and identified in the present study,and its biological characteristics were pre⁃liminarily studied,which provides a theoretical basis for the prevention and treatment of cold⁃water fish fungus.关键词
山女鳟/水霉/分离鉴定/生物学特性Key words
Oncorhynchus masou masou/Saprolegnia sp./identification/biological characteristics分类
农业科技引用本文复制引用
甄珍,王荻,刘红柏,范兆廷..山女鳟水霉病病原的分离鉴定及其生物学特性[J].江西农业大学学报,2015,(2):333-338,6.基金项目
国家公益性行业（农业）科研专项经费 （）
