江西农业大学学报Issue(3)：475-483,9.DOI:10.13836/j.jjau.2015074
赣南毛竹根际土壤微生物群落结构的PLFA分析
Phospholipid Fatty Acid Analysis of Microbial Community Structure in Rhizosphere Soil of Phyllostachys edulis in Gannan
摘要
Abstract
The rhizosphere soil microorganism which plays an important role in nutrient cycling and de-composition of organic matter,is the most active component in soil ecosystem and important in rhizosphere mi-croecological system.Rhizosphere is the micro area in which soil,plant roots and microbes interact together.By using phospholipid fatty acid ( PLFA) analysis the characteristics of soil microbial community structure in rhi-zosphere soil of P.edulis in Gannan were studied.The results showed that the content of microbes PLFA was 3.97±0.51μg/g.The content of bacteria PLFA (2.74±0.35 μg/g) was the highest among different microbe flora,followed by fungi (0.48±0.07 μg/g) and actinomycetes (0.33±0.05 μg/g).The structure patterns of soil PLFA-type contents was not affected by age,but the contents of each PLFA-type,microbe flora,F/B and SAT/MONO increased first and then decreased with age increasing.The content in rhizosphere soil of II degree bamboo was the highest,while 20∶4ω6,9,12,15c was the highest in theⅢdegree.The trend of cy/pre was in the contrast.The contents of total PLFA,fungi and G-was significantly different among different ages.The mi-crobial community structure in rhizosphere soil among different ages was different and the structure of Ⅱ de-gree was obviously different from that of other ages.i14∶0,a15∶0,cy17∶0 and 18∶1ω7c which were the bacteria biomarkers had a largest contribution to principal component 1,while 20∶4 ω6,9,12,15c ( protozoa biomark-er),18∶1ω9c and 18∶2 ω6,9c ( fungi biomarkers) and i16∶0 ( bacteria biomarker) mainly contributed to principal component 2.The reason for the above changes may be the different bamboo rhizomes and root activity in different ages.Unlike the microbial community of other ages,that of the Ⅱdegree may be more sensitive to soil environment changes,so it may be available to indicate environmental status of bamboo forest.关键词
根际/微生物群落结构/磷脂脂肪酸/毛竹/赣南Key words
rhizosphere/microbial community structure/PLFA/P.edulis/Gannan分类
农业科技引用本文复制引用
张文元,郭晓敏,胡冬南,盛可银,范承芳,刘顺,陈淼淼,钟石泉,温卫华,吴珍花,涂淑萍..赣南毛竹根际土壤微生物群落结构的PLFA分析[J].江西农业大学学报,2015,(3):475-483,9.基金项目
江西省教育厅重点科研项目（ GJJ09166）、江西省科技厅项目（20132BAB204026）、教育部博士点基金项目（20113603120004）、江西省科技落地项目（KJLD13024）和江西省中央财政项目（JXTG［2013］06）资助 （）
