江西农业大学学报Issue(3)：504-511,8.DOI:10.13836/j.jjau.2015078
因传染性胃肠炎死亡的转基因克隆仔猪的临床病理分析和初步分子鉴定
Clinic,Pathological Anatomy Symptoms and Preliminary Molecular Identification of Cloned Transgenic Pigs Dying of Transmissible Gastroenteritis
摘要
Abstract
In this study,the clinical and pathological symptoms of 6 cloned transgenic Large White dying of transmissible gastroenteritis were recorded,and the organ coefficients of multiple tissues between these trans-genic pigs and normal Large White pigs were compared.Conventional PCR and Long PCR were performed to amplify the exogenous pEF-BMPRIB ( CDS)-Neo vector, and quantitative real-time PCR ( qRT-PCR) was used to detect the copy numbers of exogenous NEO by absolute quantification assay in these transgenic pigs. The results showed that all of the sick cloned transgenic piglets exhibited diarrhea and stool abnormality,and some had fever and cough.The dissection revealed that all these pigs had intestine swelling and milk curd in stomach,and some piglets showed congestion in small intestine.Organ coefficient analyses indicated that no sig-nificant difference was found in the detected tissues ( P>0.05) except the kidney ( P<0.05) between transgen-ic and non-transgenic pigs.Molecular identification revealed that 6 cloned transgenic pigs were transgenic posi-tive individuals,whereas with loss or disruption of exogenous vector at different positions.Of the 6 transgenic pigs,4 lost or had disruptions in the promoter or CDS regions.The copy numbers of exogenous NEO in the 6 transgenic pigs were 1,2,2,3,4 and 5.In conclusion,cloned transgenic Large White piglets dying of transmissi-ble gastroenteritis showed no other abnormalities except the typical clinic and pathological anatomy symptoms of this disease.Only slight difference was found in the organ coefficients between cloned transgenic and normal Large White piglets.There was no evidence that transgenic or cloning techniques led to significant changes in recipient piglets.Molecular identification indicates that these cloned pigs are transgenic positive individuals,but the exogenous gene did not completely integrate into the host genome.关键词
转基因克隆/大白猪/分子鉴定/临床和病理症状/脏器系数Key words
cloned transgenic/large white pigs/molecular identification/clinic and pathological anatomy symptoms/organ coefficient分类
农业科技引用本文复制引用
赵雪艳,崔磊磊,任军,幸宇云,洪渊,赵科伟,王小鹏,杨强,王成斌,何小芳,邱青松,程玉柱..因传染性胃肠炎死亡的转基因克隆仔猪的临床病理分析和初步分子鉴定[J].江西农业大学学报,2015,(3):504-511,8.基金项目
转基因生物新品种培育重大专项（2011ZX08006-005，2013ZX08006-005）和江西省科技支撑计划（20111BBF60018）项目 （）
