江西农业学报Issue(7)：87-91,5.
奶牛乳房炎源性金黄色葡萄球菌分离株N2的耐药性分析及其基因特征
Drug-resistance Analysis and Genic Characteristics of Staphylococcus aureus Strain N2 Isolated from Bovine Mastitis
摘要
Abstract
In this study, Staphylococcus aureus strain N2 was isolated from bovine subclinical mastitis , and its drug-resistance was analyzed by using disk diffusion method .The biofilm-forming capacity of N2 was detected by Congo Red Agar (CRA), and eight biofilm-associated genes and nine staphylococcal enterotoxin genes were amplified by using PCR method .The results showed that N2 had wide resistance to drugs , and it was highly sensitive only to Oxacillin .N2 had stronger ability to produce biofilm , and carried all detected biofilm -associated genes ( except for bap) and 4 enterotoxin genes ( sea, sec, seg, sei) .These results suggest that there probably are several invasive means into mammary epithelia tissue for N 2 , so it is difficult to prevent and control this strain.This study can lay the foundation for the further researches of Staphylococcus aureus strain N2 .关键词
奶牛乳房炎/金黄色葡萄球菌/耐药性/生物被膜/肠毒素基因Key words
Bovine mastitis/Staphylococcus aureus/Drug-resistance/Biofilm/Enterotoxin gene分类
农业科技引用本文复制引用
徐瑾,辛向东,马梅燕,陈宏伟,张秀萍,贺建忠..奶牛乳房炎源性金黄色葡萄球菌分离株N2的耐药性分析及其基因特征[J].江西农业学报,2014,(7):87-91,5.基金项目
塔里木畜牧科技兵团重点实验室开放课题（HS201204）；国家大学生创新创业训练计划项目（TDGCX201224）；塔里木大学大学生创新创业训练项目（2013036，2013037）。 （）
