江西农业学报Issue(7)：115-117,3.
棕榈科植物 DNA的提取及 SRAP 引物筛选
Extraction of Genomic DNA from Palm Plants and Screening of SRAP Primers
摘要
Abstract
The genomic DNAs were extracted from palm plants by using CTAB method , and 100 pairs of SRAP primers were screened for clear bands and rich polymorphism by using the extracted genomic DNAs from four palm plants as templates .Finally, 14 pairs of SRAP primers were screened out and were used as the core primers of palm plants SRAP molecular markers , which pro-vided the basis for the subsequent SRAP analysis in palm plants .This study also optimized the amplification program of SRAP -PCR, which could save much experimental time on the premise of not affecting the amplification effect .关键词
棕榈科植物/DNA提取/引物筛选Key words
Palm plants/DNA extraction/Primer screening分类
农业科技引用本文复制引用
林方养,杨耀东,万婕,唐嘉,马子龙,吴翼..棕榈科植物 DNA的提取及 SRAP 引物筛选[J].江西农业学报,2014,(7):115-117,3.基金项目
海南省自然科学基金（312039）。 （）
