江西农业学报Issue(9)：90-93,4.
不同激活剂对兔卵母细胞孤雌激活效果的研究
Study on Effect of Different Stimuli on Parthenogenetic Activation of Rabbit Oocytes
摘要
Abstract
The objective of this study is to seek a stable and effective method for the parthenogenetic activation of rabbit oo -cytes, and make an experimental foundation for the somatic cell nuclear transfer of rabbit .Cumulus-oocyte complexes were harves-ted from the ovaries of superovulated rabbits , and were matured in vitro for 22~24 h;after removing the cumulus cells , the oocytes were activated by various stimuli for different time .After in vitro culture for 5~7 d, the parthenogenetic development of stimulated oocytes was assessed by microscopic observation .The results showed that rabbit oocytes could be activated not only by ionomycin treatment for 5 min, but also by ethanol treatment for 7 min, but their cleavage rate and blastula rate all were relatively low .When 6-DMAP combined with ionomycin or ethanol was used to activate rabbit oocytes , the cleavage rate (73.7%) in the treatment of ionomycin +6-DMAP was significantly (P<0.05) higher than that (38.5%) in the treatment of ethanol +6-DMAP; the treatment of ionomycin +2.0 mmol/L 6-DMAP for 5.0 h obtained the highest cleavage rate and blastula rate , being 75.6%and 45.5%respectively.It was concluded that activation method had great influence on the parthenogenetic activation rate of rabbit oo -cytes, and there were significant differences in activation effect among different treatments (combined utilization of different stimuli , various activation time ) .关键词
兔卵母细胞/孤雌激活/离子霉素/乙醇/6-二甲基氨基嘌呤Key words
Rabbit oocytes/Parthenogenetic activation/Ionomycin/Ethanol/6-DMAP分类
农业科技引用本文复制引用
王鸽,张君涛,张胜军,魏莉莉,曹书郡,徐秋勤,柳文龙,刘童,张志平..不同激活剂对兔卵母细胞孤雌激活效果的研究[J].江西农业学报,2014,(9):90-93,4.基金项目
国家自然科学基金（31001096）；河南农业大学雏鹰农牧大学生创新创业基金。 （）
