江西农业学报Issue(12):54-58,5.
柳叶马鞭草繁殖技术研究初报
Preliminary Study on Propagation Technique of Verbena bonariensis
摘要
Abstract
In order to enrich the reproduction method of Verbena bonariensis, the author treated the seeds with gibberellin ( GA) , treated the cuttings with ABT , and screened out the optimum medium formula for the tissue culture and rapid propagation of Verbena bonariensis.The results showed that:(1) GA could improve the seed vigor of Verbena bonariensis, and the germination in-dex and vitality index of 3 varieties in the treatment were higher than those in the control .There were differences in promoting effects of GA among different varieties .(2) ABT had no significant influence on the survival rate of Verbena bonariensis cuttings, but it had significant promotive effect on the root number and root length of cuttings , and the treatment with 500 mg/L ABT had the best pro-motive effect.(3) There were differences in optimum medium formula among various tissue -culture stages, the optimum medium for inducement was MS +6-BA 0.6 mg/L, the best medium for propagation was MS +6-BA 0.5 mg/L+NA A 0.1 mg/L, and the optimum medium for rooting was MS +IBA 0.4 mg/L.The highest rooting rate of Verbena bonariensis reached 93%, and the multiplication coefficient reached 4.2.关键词
柳叶马鞭草/播种繁殖/扦插/组培快繁Key words
Verbena bonariensis/Seeding propagation/Cutting/Tissue culture and rapid propagation分类
农业科技引用本文复制引用
陈华玲,谢小群,彭玉辅,赵华,彭火辉,管帮富,高柱,彭华,王小玲,黎小萍..柳叶马鞭草繁殖技术研究初报[J].江西农业学报,2014,(12):54-58,5.基金项目
江西省科技支撑计划项目(20133BBG70013);江西省科学院科研开发专项(2012-YYB-03;2014);江西省科学院省级重点实验室开放基金项目(2012-KLB-1、2012-KLB-3)。 ()