江西农业学报Issue(6):1-6,6.
辣椒种间杂交花粉母细胞减数分裂行为研究
Research on Meiosis of Pollen Mother Cells in Pepper Interspecific Hybrids
摘要
Abstract
The research on the meiosis behavior of pollen mother cells in pepper interspecific hybrids can help breeders to un-derstand the microcosmic developmental behavior of pepper and to grasp the definite reasons for pepper interspecific hybrid sterility. In this experiment, the improved chromosome microslide-preparing method was used to observe 486 pepper pollen mother cells from prophaseⅠto quartet stage.It was found that the cytokinesis of pepper pollen mother cells in meiosis behavior was of simultaneous type, and the chromosomes had multiple abnormal behaviors.Chromosome hysteresis occurred in 23.8%of metaphase-Ⅱ cells.In addition, a small quantity of chromosomal abnormal behaviors existed in the cells at prophaseⅠ, metaphaseⅠ, and anaphaseⅠ. The probability of producing micronuclei in the cells at anaphaseⅡwas 7.0%.This result can better explain why pepper interspecif-ic hybrid had low pollen fertility and low seed-setting rate, and can provide a theoretical basis for the further creation of new pepper germplasms.关键词
辣椒/种间杂交/花粉母细胞/减数分裂Key words
Pepper/Interspecific hybridization/Pollen mother cell/Meiosis分类
农业科技引用本文复制引用
刘娜,刁卫平,郭广君,潘宝贵,戈伟,许亚池,王述彬,刘金兵..辣椒种间杂交花粉母细胞减数分裂行为研究[J].江西农业学报,2015,(6):1-6,6.基金项目
国家科技支撑计划项目(2012BAD02B02);江苏省农业科技自主创新基金项目[CX(12)1004];现代农业产业技术体系建设专项资金( CARS-25)。 ()