江西农业学报Issue(7)：104-106,110,4.
猪卵母细胞体外受精影响因素的研究
Study on Factors Affecting in-vitro Fertilization of Porcine Oocytes
摘要
Abstract
The effects of caffeine concentration in fertilization medium mTBM , sperm floating capacitation time, and sperm-oo-cyte co-incubation time on the in-vitro fertilization and subsequent embryonic development of porcine oocytes were explored .The re-sults showed that adding different concentrations of caffeine into mTBM had no significant effect on the blastocyst rate of pig oocytes after in-vitro fertilization, but the fertilized cleavage rate in 3 mmol/L caffeine supplement group was significantly (P<0.05) higher than that in 5 mmol/L and 0 mmol /L caffeine supplement groups .When the time of sperm floating capacitation was lengthened , both the cleavage rate and the blastocyst rate of fertilized ovum increased first and then decreased , and the cleavage rate and blastocyst rate in 1.0 h floating group were significantly (P<0.05 ) higher than those in 1.5 h and2 .0 h floating groups.The time of sperm-oo -cyte co -incubation had no significant effect on the fertilized cleavage rate , while the blastocyst rate in 9 h co-incubation group was significantly lower than that in 3 h and 6 h co-incubation groups.关键词
猪/卵母细胞/体外受精/影响因素Key words
Pig/Oocyte/In-vitro fertilization/Influencing factors分类
农业科技引用本文复制引用
张立苹..猪卵母细胞体外受精影响因素的研究[J].江西农业学报,2015,(7):104-106,110,4.基金项目
转基因生物新品种培育重大专项（2014ZX08006－003、2014ZX08010－3）；湖北省农业科技创新中心（2011－620－001－003）；湖北省科技支撑计划（2014BBB010）。 （）
