摘要
Abstract
ObjectiveTo investigate the enzyme-linked immunoassay (ELISA) in low concentration effect of HBsAg detection.Methods In January 2014~December 2014 at our hospital outpatient and hospitalized patients in 53 patients with progress ELISA method to detect HBsAg positive and S/CO specimens of 10 or less as the research object, for all specimens using HBsAg conifrm kit as wel as supporting the use of ELISA method for testing, comparing test results.Results Kehua ELISA detected 53 cases of HBsAg positive patients, conifrmed kit and supporting by ELISA. Positive results of 45 cases (84.9%), 8 cases (15.1%) of the validation test were negative, and the progress reagent of retest results are negative. Conclusion HBsAg ELISA method for low concentration samples recheck, and neutralization test to remove false positive results, can effectively avoid the report error rate, reduce the surgery, blood transfusion and organ transplantation and other security hidden danger.关键词
酶联免疫法/表面抗原/低浓度/中和试验/Cut off值Key words
ELISA/HBsAg/Low concentration/Neutralization test/Cut off values分类
医药卫生
