生物灾害科学Issue(4)：308-313,6.DOI:10.3969/j.issn.20953704.2014.04.008
酶抑制法测定多种有机磷农药残留试验
The Test of Various Organophosphate Pesticides with Enzyme Inhibition Method
罗俊霞 1樊彦超 1宋志强 2肖海娜 1赵建波3
作者信息
- 1. 郑州市农产品质量检测流通中心，河南 郑州 450006
- 2. 巩义市夹津口镇人民政府，河南 巩义 451274
- 3. 郑州市园艺工作站，河南 郑州 450006
- 折叠
摘要
Abstract
The detection limits of 50 organophosphate pesticides were determined by means of enzyme inhibition method, and the impairment test of enzyme activity was also done. The results showed:The detection limits of DDVP and paraoxon were low; the detection limits of mevinphos, dibrom, quinalphos, dipterex, phoxim, dichlofenthion, methamidophos, and chlorpyrifosmethyl were relatively higher;the detection limits of the others were much higher. It was also found that the activity of the madeup enzyme stored in refrigerator with 9℃temperature didn’t reduce as time went by, and in every test, the activity of the enzyme had certain difference. At last, the repeatability of the determination of pesticides was bad when tested by means of enzyme inhibition method, and the determination results were also influenced by the concentration of the extracts which reacted with enzyme, chromogenic agent and substrates.关键词
酶抑制法/有机磷/检出限/酶活性/重现性Key words
enzyme inhibition method/organophosphate/detection limit/enzyme activity;repeatability分类
农业科技引用本文复制引用
罗俊霞,樊彦超,宋志强,肖海娜,赵建波..酶抑制法测定多种有机磷农药残留试验[J].生物灾害科学,2014,(4):308-313,6.
