解放军医学院学报Issue(4)：388-392,5.DOI:10.3969/j.issn.2095-5227.2015.04.023
瘦素在海人酸诱导小鼠颞叶癫痫海马损伤中的作用
Role of leptin in kainic acid-induced hippocampal injury of temporal lobe epilepsy in mice
摘要
Abstract
Objective To study the association of leptin with the occurrence and development of temporal lobe epilepsy. Methods After injection of exogenous leptin, a normal dose of kainic acid (KA, 200 ng per mouse) were microinjected into the right hippocampus of C57BL/6J mice to induce temporal lobe epilepsy. Then, seizureswere scored with the Racine scores,the hippocampal pathogenesis of mice on day 7 after the KA injection were observed, the expression level of Bax was measured by Western blot, the neuron loss of hippocampal Hilus was detected by Nissl staining, and the astrocyte activation and proliferation of hippocampal Hilus were tested by immunofluorescence method.Results Compared with KA-induced model, seizure scores increased by about 52%, the expression of Bax significantly increased by about 75%, the neuron loss significantly accelerated, and astrocyte was over activated in the KA-induced model combined with the exogenous leptin (10 mg/kg) pretreatment.Conclusion Leptin pretreatment aggravates the neuron apoptosis and pathogenesis induced by KA.关键词
瘦素/海人酸/颞叶癫痫/星形胶质细胞/神经元/海马HilusKey words
leptin/kainic acid/temporal lobe epilepsy/astrocyte/neurons/hippocampal Hilus分类
医药卫生引用本文复制引用
冯杰,邓子辉,张金英,薛辉,梁辰,李建华,颜光涛..瘦素在海人酸诱导小鼠颞叶癫痫海马损伤中的作用[J].解放军医学院学报,2015,(4):388-392,5.基金项目
科技基础性工作专项项目(2011FY130100)；国家科技支撑计划项目(2012BAK25B01) Supported by the National Key Technology R&D Program(2012BAK25B01) （2011FY130100）
