解放军医学院学报Issue(5)：502-504,509,4.DOI:10.3969/j.issn.2095-5227.2015.05.024
模拟失重对雄性大鼠背根神经节尼氏小体形态和胶质细胞源性神经营养因子的影响
Effects of simulated weightlessness on Nissl body morphology and GDNF in DRG of male rats
任宁涛 1崔赓 1张恒 1李洁 1雷伟 2刘宁 2毕龙 2吴子祥 2张然 1张永刚1
作者信息
- 1. 解放军总医院，北京 100853
- 2. 第四军医大学西京医院 骨科，陕西西安 710032
- 折叠
摘要
Abstract
Objective To investigate the effect of simulated weightlessness on dorsal root ganglia (DRG) and GDNF in rat model. Methods Eighty male Sprague-Dawley rats were randomly divided into HU group (n=40) and normal control (NC) group (n=40). The experiment lasted for 4 weeks, then L5 DRG was excised, toluidine blue staining was used to detect the changes of nissl body morphology, and GDNF was detected using immunohistochemistry method. GDNF protein expression was measured by Western-blot and GDNF mRNA expression was measured by RT-PCR.Results Nissl body staining showed that, compared with NC group, nissl bodies were stained light, smaller and scattering distribution in HU group. Immunohistochemistry revealed that the number of GDNF and IOD value reduced significantly in HU group (P<0.05). Western-blot showed that GDNF protein expression in HU group were significantly lower than NC group (P<0.05). mRNA expression of GDNF decreased significantly in HU group (P<0.05). Conclusion Four weeks of stimulated weightlessness can cause damaged changes in DRG nissl body morphology and reduction of the number, protein expression and mRNA expression of GDNF, which suggests that simulated weightlessness can cause damage changes in rat dorsal root ganglion.关键词
模拟失重/背根神经节/动物模型Key words
simulated weightlessness/dorsal root ganglia/animal model分类
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任宁涛,崔赓,张恒,李洁,雷伟,刘宁,毕龙,吴子祥,张然,张永刚..模拟失重对雄性大鼠背根神经节尼氏小体形态和胶质细胞源性神经营养因子的影响[J].解放军医学院学报,2015,(5):502-504,509,4.
