解放军医学院学报Issue(7)：738-743,6.DOI:10.3969/j.issn.2095-5227.2015.07.026
脑苷肌肽对APPswe/PS1dE9双转基因小鼠脑内β-淀粉样蛋白42和钙结合蛋白-D28K表达的影响
Effect of cattle encephalon glycoside and ignotin injection on expression of amyloidβ-peptide 42 and calbindin-D28k in APPswe/PS1dE9 transgenic mice
胡亚卓 1王鲁宁 1高雅 1韩志涛 1夏征 1耿艳 1李瑞生 2陈红艳 1王建华 1张红红1
作者信息
- 1. 解放军总医院 老年医学研究所衰老与相关疾病北京市重点实验室，北京 100853
- 2. 解放军第302医院实验动物中心，北京 100039
- 折叠
摘要
Abstract
Objective To observe the effect of cattle encephalon glycoside and ignotin injection (CEGI) on expression of amyloidβ-peptide (Aβ42) and calbindin-D28k (CB) in brain of APPswe/PS1dE9 double transgenic mice, and investigate its prevention and treatment for Alzheimer's disease. Methods A total of 48 male homozygous APPswe/PS1dE9 double transgenic mice were randomly divided into four groups, 12 in each group:low-dose CEGI group (Tg+CEGI-L;intraperitoneal injection of 6.6 ml/(kg · d) CEGI), high-dose CEGI group (Tg+CEGI-H;intraperitoneal injection of 13.2 ml/(kg · d) CEGI), positive control Donepezil group (Tg+Donepezil; intragastric administration of 2 mg/ (kg · d) Donepezil), Tg group (intraperitoneal injection of equal volume of 0.9% saline). Another 12 non-transgenic (nTg) wild-type littermates were served as normal control group (nTg, intraperitoneal injection of equal volume of 0.9%saline). After one month of drug administration, Morris water maze test was used to assess the ability of learning and memory of the mice, and the changes of expression of Aβ42 in cortex were detected by Thioflavin-S and immunohistochemistry, respectively, and the changes of expression of CB in CA1 and CA3 hippocampal region were detected by immunohistochemistry. Results Compared with Tg group, the ability of learning and memory of mice in Tg+CEGI-L group improved significantly (P < 0. 05). The expression of Aβ42 in the cortex in Tg+CEGI-L group and Tg+CEGI-H group was significantly lower than that of Tg group (43.00±10.03 vs 24.63±10.61, 24.89± 6.81, all P < 0.05). The expression of CB in CA1 and CA3 hippocampal region was higher in Tg+CEGI-L group than in Tg group (0.056±0.023 vs 0.031±0.001, P<0.05). Conclusion CEGI can reduce the expression of Aβ42 in the cortex and increase the expression of CB in CA1 hippocampal regionin APPswe/PS1dE9 double transgenic mice and improve their ability of learning and memory.关键词
脑苷肌肽/阿尔茨海默病/APPswe/PS1dE9双转基因小鼠/β-淀粉样蛋白42/钙结合蛋白-D28KKey words
cattle encephalon glycoside and ignotin injection/Alzheimer's disease/APPswe/PS1dE9 transgenic mice/amyloidβ-peptide 42/calbindin-D28k分类
医药卫生引用本文复制引用
胡亚卓,王鲁宁,高雅,韩志涛,夏征,耿艳,李瑞生,陈红艳,王建华,张红红..脑苷肌肽对APPswe/PS1dE9双转基因小鼠脑内β-淀粉样蛋白42和钙结合蛋白-D28K表达的影响[J].解放军医学院学报,2015,(7):738-743,6.
