检验医学与临床Issue(12)：1595-1598,4.DOI:10.3969/j.issn.1672-9455.2014.12.002
基因芯片在分枝杆菌菌种鉴定及结核耐药基因检测的诊断价值
Diagnostic value of gene chip in identification of mycobacterium and detection of drug-resistant genes
摘要
Abstract
Objective To investigate the diagnostic value of gene chip in identification of mycobacterium and detection of drug-resistant genes .Methods Gene chip technology was used in DNA detection of sputum ,urine ,pleu-ral effusion ,cerebrospinal fluid ,and puncture fluid samples from suspected tuberculosis and nontuberculous mycobac-teria patients ,and positive samples of mycobacterium were detected for drug-resistant genes of mycobacterium to ri-fampin and isoniazid further .Results Among 91 samples detected ,16 cases of mycobacterium tuberculosis complex , 2 cases of nontuberculous mycobacteria (1 case of Gordon′s bacillus ,1 case of mycobacterium fortuitum ) ,the positive rate of mycobacterium was 19 .8% (18/91) ,which the mycobacterium tuberculosis accounted for 17 .6% (16/91);Mycobacterium non tuberculosis accounted for 2 .2% (2/91) .The positive rate of various types of samples were dif-ferent ,which puncture pus ,sputum ,pleural effusion ,cerebrospinal fluid and urine accounted for 33 .3% ,24% , 18 .8% ,16 .7% and 15 .8% .57 patients were detected by the gene chip method and mycobacterium smear acid-fast staining at the time ,the chip was positive in 16 cases ,the positive rate was 28 .1% (16/57) ,smear was positive in 3 cases ,the positive rate was 5 .3% (3/57) ,there was significant difference between the two methods (P<0 .01) .18 ca-ses of Mycobacterium positive samples were detected for drug-resistant genes further ,4 cases showed drug-resistant genes mutation ,2 cases(1 case Gordon mycobacteria) were ropB gene 526 C → G mutation ,1 case was ropB gene 531 C → T mutation ,1 case was InhA gene promoter-15 C → T mutation .Multiple resistant strains were not detected . Conclusion The gene chip technology can be used for mycobacterial species identification and drug-resistant genes detection In different types of samples ,which is simple and rapid in procedure and have high sensitivity and good spe-cificity .关键词
基因芯片/分枝杆菌/鉴定/耐药Key words
gene chip/mycobacterium tuberculosis/identification/drug resistance引用本文复制引用
赵连爽,代娣,陈昕,云科,王倩..基因芯片在分枝杆菌菌种鉴定及结核耐药基因检测的诊断价值[J].检验医学与临床,2014,(12):1595-1598,4.基金项目
辽宁省社会发展基金资助项目（2011225019）。 （）
