检验医学与临床Issue(16)：2207-2209,3.DOI:10.3969/j.issn.1672-9455.2014.16.007
多耐药铜绿假单胞菌抗药基因检测及对常用消毒剂的抗性分析
Analysis on the detection of resistance gene and resistance to common disinfectants of multidrug resistance pseudomonas aeruginosa
摘要
Abstract
Objective To get a general view about drug-resistant gene qacE△1-sul 1(disinfectant-sulfanila-mide compound gene) carried by multidrug resistance pseudomonas aeruginosa collected from clinic ,and the resistant level against common disinfectants .Methods PCR(polymerase chain reaction) and suspension quantitative germicidal test were selected to study resistance (killing rate) of gene qacE △1-sul 1 carried by multidrug resistance pseudo-monas aeruginosa to trichloroisocyanuric acid ,iodophor ,and glutaraldehyde .Results 6 of 10 strains of multidrug re-sistance pseudomonas aeruginosa collected from clinic were qacE △1-sul 1 positive strains .The others were negative . The killing rate of 125 mg/L trichloroisocyanuric acid affected 1 minute was 99 .63% -100 .00% .The killing rate of 250 mg/L trichloroisocyanuric acid affected 1 minute was 99 .81% -100 .00% .The killing rate of the two solutions above affected 3 minutes and 5 minutes and the solution of 500 mg/L affected 1 ,3 ,5 minutes were all 100% .The kill-ing rate of solutions with effective iodine content of 125 mg/L affected 0 .5 minute was 99 .91% -100 .00% .The so-lution above affected 1 minute and 3minute along with the 250 mg/L and 500 mg/L solutions affected 0 .5 ,1 ,3 mi-nutes ,the killing rate were all 100% .The killing rate of 250 mg/L glutaraldehyde affected 1 minute was 65 .46% -99 .75% ,that of 500 mg/L solution affected 1minute was 99 .84% -100 .00% .Both of the solutions above affected 3 minuted and 5 minutes were 100% effective .Conclusion There′s a high carrying rate of gene qacE△1-sul 1 by mul-tidrug resistance pseudomonas aeruginosa .The research shows the proper use of the disinfectants can receive a good result of killing multidrug resistance pseudomonas aeruginosa .关键词
多耐药铜绿假单胞菌/qacE△1-sul 1/悬液定量杀菌试验/杀灭率Key words
multidrug resistance pseudomonas aeruginosa/drug-resistant gene qacE△1-sul 1/suspension quantitative germicidal test/killing rate引用本文复制引用
栾耀芳,王冠,王婧男,李稳,张苗..多耐药铜绿假单胞菌抗药基因检测及对常用消毒剂的抗性分析[J].检验医学与临床,2014,(16):2207-2209,3.基金项目
中华医院感染控制研究基金资助项目（Z H Y Y 12-025）。 （）
