检验医学与临床Issue(18)：2499-2502,4.DOI:10.3969/j.issn.1672-9455.2014.18.002
北京协和医院2000～2013年血培养病原菌分布及耐药性分析
Distribution and antimicrobial resistance among blood culture pathogens in Peking Union Medical College Hospital from 2000 to2013
摘要
Abstract
Objective To investigate distribution and antimicrobial resistance among blood culture pathogens in Peking Union Medical College Hospital from 2000 to 2013 .Methods Pathogens from outpatients′and inpatients′blood cultures in this hospital were collected continuously from 2000 to 2013 .Disk diffusion method was used to de-termine the in vitro susceptibility of pathogens to antimicrobial agents .WHONET5 .6 software was used to analyze the data .Results A total of 7 199 non-repetitive blood culture pathogens were isolated from 2000 to 2013 ,excluding Corynebacteirum spp ,Propionibacterium acnes ,Micrococcus spp and Bacillus spp .Gram-negative aerobic and Faculta-tively-anaerobic pathogens accounted for 40 .6% - 54 .2% .Coagulase-negative Staphylococcus accounted for 18 .7% -35 .1% .The proportion of other gram-positive cocci ,including mainly Staphylococcus aureus ,Enterococcus spp and Streptococcus spp ,was stable ,with isolation rate of 19 .7% -25 .8% .Fungi with isolation rate of 0 .5% -5 .7% .The resistance data from 2011 to 2013 revealed that the most active antimicrobial agents against Escherichia coli and Klebsiella pneumonia were meropenem ,imipenem and ertapenem .Against Pseudomonas aeruginosa ,the sus-ceptibility rates to drugs were more than 70 .0% except aztreonam .Against Acinetobacter baumannii ,the susceptibili-ty rates to drugs were less than 25 .0% except minocycline .Against Coagulase-negative Staphylococcus and staphylo-coccus aureus ,the methicillin resistance rate was 80 .7% and 42 .3% respectively .Drugs with high activity included linezolid ,vancomycin and teicoplanin .Conclusion Gram-negative bacteria could account for major part of the blood culture pathogens ,with increasing trend in recent years in this hospital .The isolation rate of coagulase-negative staphylococcu decreased in recent five years and Candida isolation rate increased .Carbapenems might be the most ac-tive drugs against Enterobacteriaceae .Linezolid ,vancomycin and teicoplanin could be the most potent antimicrobial a-gents against Gram-positive cocci .关键词
血培养/病原菌分布/耐药性Key words
blood culture/pathogen distribution/antimicrobial resistance引用本文复制引用
张慧,杨启文,徐英春,谢秀丽,陈民钧..北京协和医院2000～2013年血培养病原菌分布及耐药性分析[J].检验医学与临床,2014,(18):2499-2502,4.基金项目
国家自然科学基金资助项目（H1908／81101287）。 （）
