检验医学与临床Issue(20)：2854-2855,2857,3.DOI:10.3969/j.issn.1672-9455.2014.20.024
HBeAg转阴与HBV基因型分布和YMDD变异位点的相关性分析
Correlation study between HBeAg-negative and HBV genotype YMDD mutations in patients with hepatitis B virus infec -tion
吴艾霖 1熊怡淞 1王艳艳 1曲远青 1段小刚 1吴丽娟1
作者信息
- 1. 成都军区总医院临床实验医学研究与保障中心，成都 610083
- 折叠
摘要
Abstract
Objective To study the correlation between hepatitis B e antigen (HBeAg)-negative and HBV gen-otype ,YMDD mutation induced by long-term use of lamivudine in patients with hepatitis B virus infection .Methods 1 200 patients with chronic hepatitis B were recruited in this study ,of which 577 patients were HBeAg negative and 623 were HBeAg positive .Enzyme-linked immunoadsordent assay(ELISA) ,Taqman QPCR and linear probe hybrid-ization were used to detect HBeAg 、HBV-DNA and HBV genotypes respectively .And the relationships between them were analyzed .Results There were 233 patients(40 .38% )with HBV-DNA virus replication (HBV-DNA ≥ 103 ) in all the 577 HBeAg-negetive patients .Further genotyping results showed that there were 163 (69 .96% ) ,57 (24 .46% ) ,11(4 .72% ) and 2(0 .86% ) cases in genotype A ,B ,C and D respectively .YMDD mutation test found that there was 106 cases in 233 patients(45 .49% )with YMDD mutation ,composed by Ⅴ mutation(68 .87% ) ,Ⅰ muta-tions(22 .64% ) ,and Ⅰ/Ⅴ symbiotic mutation(8.49% ) .In the 106 YMDD mutation cases there were 0(0 .00% ) ,72 (67 .92% ) ,27(25 .47% ) ,and 7(6 .60% )cases for genotype A ,B ,C and D respectively .Conclusion Replication of hepatitis B virus was somewhat exist in patients with HBeAg-negative .Continuous monitoring of HBV viruses copy number ,YMDD mutation and genotype is necessary for the treatment of these patients .关键词
e 抗原阴性/乙型肝炎病毒/YMDD 突变/HBV 基因型Key words
e anti-negative/hepatitis B virus/YMDD mutation/HBV genotype引用本文复制引用
吴艾霖,熊怡淞,王艳艳,曲远青,段小刚,吴丽娟..HBeAg转阴与HBV基因型分布和YMDD变异位点的相关性分析[J].检验医学与临床,2014,(20):2854-2855,2857,3.
