检验医学与临床Issue(20)：2858-2859,2.DOI:10.3969/j.issn.1672-9455.2014.20.026
核酸检测技术在献血人员血液筛查中的应用
Application of nucleic acid detection technology in blood screening of blood donors
柯苑 1赵静 1马成平 1黄敏 1姚慧兰 1张立波 1马贵明1
作者信息
- 1. 南京红十字血液中心实验室，南京 210003
- 折叠
摘要
Abstract
Objective To explore the value of nucleic acid detection technology (NAT ) for blood screening to decrease the infection induced by blood donors in the window period and infected insidiously .Methods A total of 54 358 blood samples were detected by ELISA for hepatitis B surface antigen (HBsAg) ,antibody of human immuno-deficiency virus (HIV-Ab ) ,antibody of hepatitis C virus (anti-HCV ) ,and transcription-mediated amplification (TMA)method were used to detecte the DNA of hepatitis B virus (HBV-DNA ) ,the RNA of hepatitis C virus (HCV-RNA)and the RNA of type 1 human immunodeficiency virus(HIV-1 RNA) .Results Fifty-one samples were positive by NAT ,but negative by ELISA .A total of 33 samples in these 51 samples were identified .The HBV-DNA of 32 donors were positive ,and the HIV-1 RNA of one donor was positive ,who was identified as suspected HIV-1 positive (gp160 and p24 ± ) by western blot method in Nanjing municipal center for disease control and prevention . Six months later ,this blood donor was identified as HIV-1 positive (gp160 ,gp120 ,p66 ,p51 ,gp41 ,p31 ,p24 ,p17 + ) by western blot method in Nanjing municipal center for disease control and prevention .Conclusion NAT could effec-tively decrease the infection induced by blood donors in the window period and infected insidiously ,so as to reduce the risk of blood transfusion .关键词
血液筛查/核酸检测技术/“窗口期”/转录介导扩增技术Key words
blood screening/nucleic acid detection technology/window period/transcription-mediated amplification引用本文复制引用
柯苑,赵静,马成平,黄敏,姚慧兰,张立波,马贵明..核酸检测技术在献血人员血液筛查中的应用[J].检验医学与临床,2014,(20):2858-2859,2.
