检验医学与临床Issue(z1)：56-58,3.DOI:10.3969/j.issn.1672-9455.2014.25.024
两种检测高值甲胎蛋白出现钩状效应的方法研究
Study on two different methods for detecting high value AFP inducing hook effect
贺鹃 1雷旦生 1喻晶1
作者信息
- 1. 湖北省肿瘤医院检验科，武汉 430079
- 折叠
摘要
Abstract
Objective To discuss the hook effect induced by high value Alpha fetoprotein(AFP)and its influ-ence for experimental results.Methods Seventy nine cases of high value AFP(> 105 μg/L)specimens of patients with primary liver cancer were double diluted with electrochemical luminescence method and chemiluminescence method respectively,then observed whether the hook effect take place,and the experimental results were compared and analyzed.Results The detecting results with two methods showed that the concentrations exceeded 4 ×104 μg/L yield hook effect and the barb points were above the maximum detection range(Roche Combas e601:0-1 210 μg/L;LIASON XL-100:0-1 000 μg/L).Meantime,the results in the linear range with two methods varied linearly with the effective signal values.Conclusion The hook effect was observed in the results of both methods.Moreover,the e-mergence of abnormally low value caused by the hook effect was avoided as the barb points were above the maximum detection range.It′s suggested that the specimens should be effectively diluted for the results above the maximum tes-ting range when both of the above methods are employed.And the results within the linear range could be regarded as the real results.关键词
钩状效应/甲胎蛋白/电化学发光法/化学发光法Key words
hook effect/alpha fetoprotein/electrochemiluminescence immunoassay/chemiluminescence immunoassay引用本文复制引用
贺鹃,雷旦生,喻晶..两种检测高值甲胎蛋白出现钩状效应的方法研究[J].检验医学与临床,2014,(z1):56-58,3.
