检验医学与临床Issue(z1)：233-236,4.DOI:10.3969/j.issn.1672-9455.2014.25.123
50例骨髓增生异常综合征间期荧光原位杂交分析
Detection of chromosome abnormality in 30 patients with myelodysplastic syndrome by interphase fiuorescence in situ hy-bridization
陈晓燕 1刘勇 2应逸 1杜庆华 1王顺清1
作者信息
- 1. 广州市第一人民医院血液内科中心实验室 510180
- 2. 广州金域医学检验中心，广州 510330
- 折叠
摘要
Abstract
Objective To investigate the molecular cytogenetic abnormalities in MDS by fluorescence in situ hybridization(FISH).Methods Fifty patients of MDS were enrolled in this study.Panel probes of +8、-7/7q-、-5/5q-、20q- were used to detect genetic abnormality in MDS in these patients as well as conventional chromosome karyotype.Results Overall,32 cases were found carrying chromosome abnormalities by CC and FISH,the remaining 18 cases showed normal results.CC revealed cytogenetic abnormalities in 23 of 50 cases(46%),while FISH revealed chromosome abnormalities in 31 of 50 patients(62%).There was no significant difference between the two technics (P =0.108).Conclusion FISH can sensitively detect small cloned abnormalis which is an important complement for conventional karyotype analysis.关键词
骨髓增生异常综合征/间期荧光原位杂交/细胞遗传学Key words
myelodysplastic syndrome/interphase fluorescence in situ hybridization/cytogenetics引用本文复制引用
陈晓燕,刘勇,应逸,杜庆华,王顺清..50例骨髓增生异常综合征间期荧光原位杂交分析[J].检验医学与临床,2014,(z1):233-236,4.
