检验医学与临床Issue(1)：6-8,3.DOI:10.3969/j.issn.1672-9455.2015.01.003
高效液相色谱法测定大鼠坐骨神经组织中山梨醇的浓度
Determination of sorbitol content in rat sciatic nerve tissue by HPLC
摘要
Abstract
Objective To establish a method for the determination of sorbitol content in rat sciatic nerve tissue by high‐performance‐liquid‐chromatograph(HPLC) .Methods The HPLC was performed on the Angilent ZORBAX SB C18 column(4 .6 mm × 250 mm ,5 μm) with acetonitrile(A)‐water(B) as the mobile phase for conducting the gradient elution and with L‐rhamnose as the internal standard ,at the column temperature of 25 ℃ ,the flow rate of 1 .0 mL・min-1 ,the wavelength at 228 nm and the injection volume of 20 μL .The methodological investigation of this method for determining the sorbitol content in rat sciatic nerve tissue was performed ,including the specificity test ,linear relation test ,precision test ,sample injection recovery test and the stability test .Results The linear range of sorbitol content in rat sciatic nerve was 7 .5-600 nmol・mL -1 (r2 =0 .999 9) and the lowest detection limit was 7 .5 nmol・mL -1 .The intraday and interday precision were less than 10% ,the recovery rate of sorbitol sample size was higher and met the requirement of sample recovery rate of 80% -120% ;the method had good stability ,including the repeated freezing‐thawing for 3 times ,placing in the room temperature for 4 h ,freezing preservation for 28 d at the temperature of -20 ℃ and placing of the processed sample in injector for 24 h ,RSD of each test was less than 10% .Conclusion This method is simple ,convenient ,reliable in detection results and can be used in determining the sorbitol content in sciatic nerve tissue .关键词
坐骨神经/山梨醇/L-鼠李糖/高效液相色谱法Key words
sciatic nerve/sorbitol/L-rhamnose/HPLC引用本文复制引用
杨鑫伟,年宏蕾,姚伟洁,李情琴,刘铜华,李静,张楠,许利平..高效液相色谱法测定大鼠坐骨神经组织中山梨醇的浓度[J].检验医学与临床,2015,(1):6-8,3.基金项目
国家“重大新药创制”科技重大专项（2009ZX09103-303）；北京市教育委员会科技计划面上项目（KM201410025014）；首都中医药研究专项（13ZY01）。 （）
