检验医学与临床Issue(1)：55-57,3.DOI:10.3969/j.issn.1672-9455.2015.01.021
原发性膜性肾病患者外周血淋巴细胞亚群的变化及其与蛋白尿的关系
Changes of peripheral blood lymphocyte subgroups in patients with primary membranous nephropathy and their relation- ship with proteinuria
马东红 1郭明好 1许清玉 1刘云 1刘向东 1刘丽1
作者信息
- 1. 新乡医学院第一附属医院肾内科，河南新乡 453100
- 折叠
摘要
Abstract
Objective To analyze the changes of lymphocyte subgroups in peripheral blood for the patients with primary membranous nephropathy(PMN)and their relationship with proteinuria .Methods 40 cases of PMN in the nephrology department of our hospital were selected and their detailed clinical data were collected .The peripheral blood was collected for detecting the levels of lymphocyte subgroups (CD3+ ,CD4+ ,CD8+ ,CD4+ /CD8+ ,CD16+56+ ,CD19+ ) by the flow cytometry .Simultaneous 20 healthy volunteers were selected as the control group .The differences in peripheral blood lymphocyte subgroups were compared between the PMN patients and the healthy con‐trol group .According to the proteinuria levels ,the PMN patients were divided into the massive proteinuria group (MPG ,proteinuria quantity≥3 .5 g/d) and the non‐massive proteinuria group(NMPG ,proteinuria quantity<3 .5 g/d) .The differences in peripheral blood lymphocyte subgroups were compared between the MPG group and the NMPG group and their possible clinical significance was investigated .Results (1) Compared with the healthy con‐trol group ,the CD4+ cell levels had a rising trend(51 .83 ± 6 .80 vs .43 .5 ± 5 .65 ,P>0 .05) and the CD8+ cell levels had a downward trend(28 .83 ± 5 .60 vs .35 .33 ± 5 .00 ,P>0 .05) in the PMN patients ,while the CD4+ /CD8+ ratio was significantly increased compared with the healthy control group (1 .86 ± 0 .43 vs .1 .27 ± 0 .33 ,P<0 .01) .There was no significant difference in the CD16+CD56+ count between two groups ,while the CD19+ cell count in the PMN patients was significantly increased(8 .00 ± 2 .76 vs .7 .33 ± 2 .66 ,P<0 .01) .(2) Compared with the NMPG group , the CD19+ cell count in the MPG group was significantly increased(9 .80 ± 3 .19 vs .7 .10 ± 2 .23 ,P<0 .01) ,while the CD4+ cell count ,CD8+ cell count and the CD4+ /CD8+ ratio had no statistically significant differences between the MPG and NMPG groups .Conclusion PMN patients appear obvious abnormalities of B lymphocytes and the shift of CD4+ /CD8+ cells ratio .The abnormal degree of B lymphocyte is related to proteinuria quantity .Therefore ,monito‐ring the lymphocyte subgroups changes can help to assess the disease condition and guide the treatment .关键词
原发性膜性肾病/淋巴细胞亚群/蛋白尿Key words
primary membranous nephropathy/lymphocyte subgroups/proteinuria引用本文复制引用
马东红,郭明好,许清玉,刘云,刘向东,刘丽..原发性膜性肾病患者外周血淋巴细胞亚群的变化及其与蛋白尿的关系[J].检验医学与临床,2015,(1):55-57,3.
