检验医学与临床Issue(3)：352-354,357,4.DOI:10.3969/j.issn.1672-9455.2015.03.026
短串联重复序列基因座在石蜡包埋组织中的应用
Application of short tandem repeats loci genotyping in paraffin embedded tissue
赖力 1韩莉莉 1沈晓丽1
作者信息
- 1. 福建省立医院司法鉴定所/福建省心血管病重点实验室/福建医科大学省立临床学院，福州 350001
- 折叠
摘要
Abstract
Objective To study the difference of the short tandem repeats (STR) loci genotyping in paraffin embedded tissue by using three kits .Methods DNA were extracted from intestinal tumor in paraffin‐embedded tis‐sue which was preservd for a month by using Qiagen method ,fluorescent multiplex PCR by using identifiler kit ,Pow‐erPlex 21 kit and Investigator HDplex kit ,capillary electrophoresis and fragment analysis technique were used to de‐tect STR loci .Comparing the recalling rations of STR loci of three kits and the genotype of the homologous normal samples which include paraffin embedded tissue ,blood ,hair and oral swab .Results DNA concentration extracted from paraffin‐embedded tissue were detected between 6-85 ng/μL ,OD=1 .7 -2 .2 .when DNA concentration >15 ng/μL ,the full STR loci genotype was detected by identifiler kit ,and when DNA concentration was 85 ng/μL ,the full STR loci genotype was detected by PowerPlex 21 kit ,however ,there was no full peaks of all the STR loci by using HDplex kit .There were several different STR loci genotype between paraffin embedded tissue and other homologous normal samples .There were allelic imbalance and drop‐outs in D6S474 ,D4S2366 and D21S2055 .Conclusion The concentration and purity of DNA extracted from paraffin embedded tissue were the important influential factors for STR loci genotype .The phenomenon of missing genetic information was related to sample properties and the detec‐ting system .There was high practical value for STR loci genotyping in paraffin embedded tissue by using identifiler kit .关键词
石蜡包埋组织/STR分型/个体识别Key words
paraffin embedded tissue/STR typing/individual identification引用本文复制引用
赖力,韩莉莉,沈晓丽..短串联重复序列基因座在石蜡包埋组织中的应用[J].检验医学与临床,2015,(3):352-354,357,4.
