检验医学与临床Issue(4)：436-438,3.DOI:10.3969/j.issn.1672-9455.2015.04.002
耐甲氧西林金黄色葡萄球菌MecA基因的实时荧光定量PCR方法学评价
Evaluation of FQ-PCR method for detecting methicillin-resistant Staphylococcus aureus MecA gene
摘要
Abstract
Objective To evaluate the reliability of fluorescent quantitative polymerase chain reaction (FQ‐PCR) method for detecting methicillin‐resistant Staphylococcus aureus (MRSA) MecA resistant gene .Methods A total of 125 clinical specimens were collected from June 2013 to December 2013 ,including 40 blood specimens ,52 sputum specimens ,33 specimens of wound secretions .FQ‐PCR method and bacteria identification were used to detect all the specimens ,and then analyze the consistent rate of results ,sensitivity and specifity .Results The sensitivity of FQ‐PCR method was 0 .159 pg/μL ,the specificity of FQ‐PCR in detecting bacterial strain cultured and identified was100% ,and the consistent rate with the results of clinical samples was 97 .6% .Conclusion FQ‐PCR method is more convenient ,fast in detecting MecA gene of MRSA ,and sensitivity ,specificity are ideal ,the consistent rate with bacterial identification result is high ,so could be used widely in clinical applications .关键词
耐甲氧西林金黄色葡萄球菌/M ecA基因/实时荧光定量聚合酶链反应/微生物鉴定Key words
methicillin-resistant Staphylococcus aureus/MecA gene/fluorescent quantitative polymerase chain reaction/bacterial identification引用本文复制引用
欧红玲,陈凤华,王岩,张巧云,王欣茹..耐甲氧西林金黄色葡萄球菌MecA基因的实时荧光定量PCR方法学评价[J].检验医学与临床,2015,(4):436-438,3.基金项目
全军十二五面上课题（CWS11J205）。 （）
