检验医学与临床Issue(5)：622-624,3.DOI:10.3969/j.issn.1672-9455.2015.05.017
结合雌激素对去势大鼠骨折愈合生物力学指标的影响
Effects of premarin on bone biomechanic indexes in fracture healing of ovariectomized rats
陈镘如 1徐克惠 2乔林2
作者信息
- 1. 四川省人民医院妇产科，成都 610072
- 2. 四川大学华西第二医院妇产科，成都 610041
- 折叠
摘要
Abstract
Objective To explore the effects of conjugated estrogen hormones (premarin) on the bone biome‐chanic indexes and fracture healing of the ovariectomized rats .Methods 54 female rats ,aged 6‐month‐old ,were divid‐ed into 3 groups:premarin group ,sham operation group(SHAM group) and ovariectomized group(OVX group) .Rats in the premarin group and the OVX group were ovariectomized .The right femur was broken by operation at 8 weeks after ovarrectomization .Premarin [0 .065 mg/(kg · d)] was given immediately through intragastric administration after establishment of osteporotic fracture model in the premarin group .Rats in the 3 groups were randomly killed at the end of postoperative 2 ,4 ,6 ,8 weeks respectively .The right femur was examined for observing the change of bone callus by X‐rays ;the absorbance determination of cartilage callus and bone callus in the end of femural fracture were performed;the integral absorbance detection of positive expression of type Ⅰ bone collagen and the detection of bio‐mechanical indexes of right femur at 8 weeks after fracture were performed .Results The X‐ray examination showed that the bone callus density in various periods after fracture in the premarin group and the SHAM group were signifi‐cantly higher than that in the OVX group .The absorbance of cartilage callus and bone callus of the end of fracture at 8 weeks after fracture in the premarin group were lower than that in the OVX group with statistical difference (P<0 .05) ,but without statistical differences compared with that in the SHAM group (P>0 .05) .The integral absorbance of positive expression of type Ⅰ bone collagen in vanous periods in the OVX group was significantly lower than that in the premarin group and the SHAM group(P<0 .05) ,while there were no statistical differences in the expression between the premarin group and the SHAM group(P>0 .05) .The destructive intension ,elastic modulus and stiffness coefficient of right femur in the premarin group were better than those in the OVX group with statistical differences (P<0 .05) ,while without statistical differences compared with the SHAM group (P>0 .05) .Conclusion Premarin may increase the synthesis of type Ⅰ collagen ,and the flexibility and hardness of bones ,finally improve the biome‐chanics properties .关键词
结合雌激素/绝经后骨质疏松性骨折/生物力学/大鼠Key words
conjugated estrogen hormones (premarin)/postmenopausal osteoporotic fracture/biomechan-ics/rat引用本文复制引用
陈镘如,徐克惠,乔林..结合雌激素对去势大鼠骨折愈合生物力学指标的影响[J].检验医学与临床,2015,(5):622-624,3.
