摘要
Abstract
Objective To analyze the relationship between the particulate chromatography and the high per‐formance liquid chromatography (HPLC) in detecting glycosylated hemoglobin (HbA1c) and their clinical conform‐ance situation .Methods According to the Clinical and Laboratory Standard Institute (CLSI) EP9‐A2 document ,8 blood samples per day were randomly extracted for 5 consecutive days ,a total of 40 samples were collected .The HbA1c in each sample was detected by the particle chromatography and HPLC respectively ,and the detection data were performed the comparative analysis .Results The comparative analysis showed that the two systems had was repeatability and could meet the criteria of the related laboratory tests .The outlier values were not found .The equa‐tion of linear regression was Y=1 .088 5X-0 .521 4 ,the correlation coefficient r=0 .993 ,95% confidence interval :(B^C ,low ,B^C ,high )was (0 .019 1 ,0 .181 8) ,which was less than the allowable error (-0 .35 ,0 .35) .There were no signif‐icant differences in the determination results of HbA1c between the two methods .No meaningful bias was found . Conclusion The particulate chromatography and HPLC could have high comparability and good correlation ,and could meet the clinical demand .The clinical laboratory could choose the appropriate method according to its own con‐ditions .关键词
糖化血红蛋白/相关性/微粒色谱法/高效液相色谱法Key words
glycosylated hemoglobin/correlation/particulate chromatography/high performance liquid chromatography
