检验医学与临床Issue(7)：897-899,3.DOI:10.3969/j.issn.1672-9455.2015.07.008
脂肪乳输注对血红蛋白测定的影响研究
Influence of fat emulsion infusion on determination of hemoglobin
摘要
Abstract
Objective To study the influence of fat emulsion infusion on the detection of hemoglobin (Hb) and related indexes .Methods Ten cases of volunteers receiving fat emulsion infusion were selected and taken 2 mL ve‐nous blood ,at 1 ,2 ,4 ,8 and 12 h after infusion .Plasma levels of triglyceride (TG) and Hb were detected .Then ,plas‐ma samples of the five time points were prepared .One healthy volunteer with blood type of O was selected and taken 6 mL venous blood .Condense red blood cells (RBC) suspension was prepared by removing plasma .Then ,200 μL of 1 ,2 ,4 ,8 and 12 h mixed chylous plasma was mixed with 200 μL healthy condense RBC suspension respectively ,and 200 μL healthy condense RBC suspension was mixed with 200 μL normal saline to prepared control tube .One volun‐teer with low pigment anemia and blood type O was selected and taken 6 mL venous blood .Condense low pigment RBC suspension was prepared .Then ,100 μL condense low pigment RBC suspension was mixed with 100 ,200 ,300 and 400 μL chylous plasma ,TG concentration of which was 15 .23 mmol/L ,and 100 μL condense low pigment RBC suspension was mixed with 100 μL normal saline to prepare control tube .Levels of Hb and related indicators of each tubes were detected by using Mindray BC‐6800 analyzer .Every indicator was detected for twice ,and average was cal‐culated .Results Plasma levels of TG rapidly increased after fat emulsion infusion ,and the peak value was 25 .89 mmol/L .The peak plasma levels of Hb was 19 g/L .The influences of chylous plasmas of different time points on lev‐els of RBC ,haematocrit (HCT ) and mean corpuscular volume (MCV ) were not obvious ,but the false increase of Hb ,mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) ,especially with 4 h after infusion .With the increasing of severity of anemia ,the degree of influences on Hb ,MCH and MCHC were more obvious .Fat emulsion was with little influence on Hb and related indexes ,12 h after infusion .Conclusion Hb , MCH and HCHC should not be detected within 4 h after fat emulsion infusion ,especially in volunteer with severe a‐nemia .Results detected 12 h after infusion might be more reliable .关键词
脂肪乳/血红蛋白/血细胞分析Key words
fat emulsion infusion/hemoglobin/haemocyte analysis引用本文复制引用
黄浩,黄玲莎,朱波,梁艺华,任宁毅,徐茜..脂肪乳输注对血红蛋白测定的影响研究[J].检验医学与临床,2015,(7):897-899,3.基金项目
广西壮族自治区卫生厅自筹经费科研课题（Z2014587）。 （）
